Wenyang Lishui Decoction Ameliorates Podocyte Injury in Membranous Nephropathy Rat and Cell Models by Regulating p53 and Bcl-2
المؤلفون المشاركون
Lu, Huan
Luo, Yuezhong
Su, Baolin
Tang, Shuifu
Chen, Gangyi
Zhang, Licai
Song, Chao
Wang, Chao
Tu, Haitao
Wu, Xinbo
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-05-13
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Wenyang Lishui decoction (WYD) has been frequently used to treat patients with membranous nephropathy (MN) in China.
Our previous study in vitro showed that WYD aqueous extract could alleviate F-actin reorganization of podocytes induced by serum from idiopathic membranous nephropathy (IMN) patients.
This study aims to investigate the effects and molecular mechanisms of WYD on MN.
MN rat models were induced by cationic bovine serum albumin.
Experimental rats were divided into four groups: normal, model, WYD, and benazepril.
The normal group consisted of normal rats receiving distilled water for four weeks, while the model, WYD, and benazepril groups consisted of MN rats receiving distilled water, 16.5 g/kg/day WYD aqueous extract, and 10 mg/kg/day benazepril, respectively.
Alanine aminotransferase, kidney function, albumin, and 24 h urine total protein (UTP) were measured.
Hematoxylin-eosin and electron microscopy analyses were performed.
Mouse podocytes were induced to develop cell models by serum from IMN patients with antibody to the M-type phospholipase A2 receptor and spleen and kidney Yang deficiency syndrome.
They were divided into five groups: control, model, 2 mg/ml WYD, 4 mg/ml WYD, and 8 mg/ml WYD.
CCK-8 assays, flow cytometry, qRT-PCR, and Western blot analyses were performed.
In the animal experiment, side effects of WYD were not found.
Also, there was no significant difference in kidney function among the groups.
In addition, UTP level was significantly reduced, and kidney histological damage was restored in both WYD and benazepril groups but difference in UTP level between them was not found.
In the cell experiment, apoptosis rate was increased in the model group while it was decreased by coincubation with WYD.
Besides, mRNA and protein levels of p53 were decreased, and those of Bcl-2 were increased by treatment using WYD.
In conclusion, WYD could reduce proteinuria and ameliorate podocyte injury by regulating the expression of p53 and Bcl-2.
The study is registered in the Chinese Clinical Trial Registry (ChiCTR-OCH-14005137).
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lu, Huan& Luo, Yuezhong& Su, Baolin& Tang, Shuifu& Chen, Gangyi& Zhang, Licai…[et al.]. 2020. Wenyang Lishui Decoction Ameliorates Podocyte Injury in Membranous Nephropathy Rat and Cell Models by Regulating p53 and Bcl-2. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1157105
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lu, Huan…[et al.]. Wenyang Lishui Decoction Ameliorates Podocyte Injury in Membranous Nephropathy Rat and Cell Models by Regulating p53 and Bcl-2. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1157105
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lu, Huan& Luo, Yuezhong& Su, Baolin& Tang, Shuifu& Chen, Gangyi& Zhang, Licai…[et al.]. Wenyang Lishui Decoction Ameliorates Podocyte Injury in Membranous Nephropathy Rat and Cell Models by Regulating p53 and Bcl-2. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1157105
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1157105
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر