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Deciphering the Molecular Targets and Mechanisms of HGWD in the Treatment of Rheumatoid Arthritis via Network Pharmacology and Molecular Docking
المؤلفون المشاركون
Fan, Yi hua
Wang, Aihua
Zeng, Ping
Liu, Wei
Tian, Chunying
Jin, Yue
Du, Shaopeng
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-26
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Background.
Huangqi Guizhi Wuwu Decoction (HGWD) has been applied in the treatment of joint pain for more than 1000 years in China.
Currently, most physicians use HGWD to treat rheumatoid arthritis (RA), and it has proved to have high efficacy.
Therefore, it is necessary to explore the potential mechanism of action of HGWD in RA treatment based on network pharmacology and molecular docking methods.
Methods.
The active compounds of HGWD were collected, and their targets were identified from the Traditional Chinese Medicine Systems Pharmacology Database (TCMSP) and DrugBank database, respectively.
The RA-related targets were retrieved by analyzing the differentially expressed genes between RA patients and healthy individuals.
Subsequently, the compound-target network of HGWD was constructed and visualized through Cytoscape 3.8.0 software.
Protein-protein interaction (PPI) network was constructed to explore the potential mechanisms of HGWD on RA using the plugin BisoGenet of Cytoscape 3.8.0 software.
Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) were performed in R software (Bioconductor, clusterProfiler).
Afterward, molecular docking was used to analyze the binding force of the top 10 active compounds with target proteins of VCAM1, CTNNB1, and JUN.
Results.
Cumulatively, 790 active compounds and 1006 targets of HGWD were identified.
A total of 4570 differentially expressed genes of RA with a p value <0.05 and log 2fold change > 0.5 were collected.
Moreover, 739 GO entries of HGWD on RA were identified, and 79 pathways were screened based on GO and KEGG analysis.
The core target gene of HGWD in RA treatment was JUN.
Other key target genes included FOS, CCND1, IL6, E2F2, and ICAM1.
It was confirmed that the TNF signaling pathway and IL-17 signaling pathway are important pathways of HGWD in the treatment of RA.
The molecular docking results revealed that the top 10 active compounds of HGWD had a strong binding to the target proteins of VCAM1, CTNNB1, and JUN.
Conclusion.
HGWD has important active compounds such as quercetin, kaempferol, and beta-sitosterol, which exert its therapeutic effect on multiple targets and multiple pathways.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Wei& Fan, Yi hua& Tian, Chunying& Jin, Yue& Du, Shaopeng& Zeng, Ping…[et al.]. 2020. Deciphering the Molecular Targets and Mechanisms of HGWD in the Treatment of Rheumatoid Arthritis via Network Pharmacology and Molecular Docking. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1157174
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Wei…[et al.]. Deciphering the Molecular Targets and Mechanisms of HGWD in the Treatment of Rheumatoid Arthritis via Network Pharmacology and Molecular Docking. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1157174
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Wei& Fan, Yi hua& Tian, Chunying& Jin, Yue& Du, Shaopeng& Zeng, Ping…[et al.]. Deciphering the Molecular Targets and Mechanisms of HGWD in the Treatment of Rheumatoid Arthritis via Network Pharmacology and Molecular Docking. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1157174
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1157174
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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