Huang-Pu-Tong-Qiao Formula Ameliorates Tau Phosphorylation by Inhibiting the CaM-CaMKIV Pathway
المؤلفون المشاركون
Shen, Guo-Ming
Ye, Shu
Cai, Biao
Zhou, Peng
Wang, Guoquan
Gao, Huawu
Hua, Rupeng
You, Liangzhen
Yao, Yongchuan
Wang, Yan
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-05-12
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Alzheimer’s disease (AD) is a complex neurodegenerative disease.
It is a chronic, lethal disease in which brain function is severely impaired and neuronal damage is irreversible.
Huang-Pu-Tong-Qiao (HPTQ), a formula from traditional Chinese medicine, has been used in the clinical treatment of AD for many years, with remarkable effects.
However, the neuroprotective mechanisms of HPTQ in AD have not yet been investigated.
In the present study, we used AD models in vivo and in vitro, to investigate both the neuroprotective effect of HPTQ water extracts (HPTQ-W) and the potential mechanisms of this action.
For the in vivo study, after HPTQ intervention, the Morris water maze test was used to examine learning and memory in rats.
Transmission electron microscopy and immunofluorescence methods were then used to investigate neuronal damage.
For the in vitro experiments, rat primary hippocampal neurons were cultured and cell viability was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.
Additionally, mRNA levels of CaM, CaMKK, CaMKIV, and tau were examined using qRT-PCR, and protein expression of CaM, CaMKK, p-CaMKIV, and p-tau were examined using western blot.
In vivo, we revealed that HPTQ significantly improved learning and memory deficits and attenuated neuronal damage in the AD rat model.
Furthermore, in vitro results showed that HPTQ significantly increased cell viability in the AD cell model.
We also demonstrated that HPTQ significantly decreased the mRNA levels of CaM, CaMKK, CaMKIV, and tau and significantly decreased the protein expressions of CaM, CaMKK, p-CaMKIV, and p-tau.
In conclusion, our results indicated that HPTQ improved cognition and ameliorated neuronal damage in AD models and implicated a reduction in tau phosphorylation caused by inhibition of the CaM-CaMKIV pathway as a possible mechanism.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ye, Shu& Cai, Biao& Zhou, Peng& Wang, Guoquan& Gao, Huawu& Hua, Rupeng…[et al.]. 2020. Huang-Pu-Tong-Qiao Formula Ameliorates Tau Phosphorylation by Inhibiting the CaM-CaMKIV Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1158273
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ye, Shu…[et al.]. Huang-Pu-Tong-Qiao Formula Ameliorates Tau Phosphorylation by Inhibiting the CaM-CaMKIV Pathway. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1158273
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ye, Shu& Cai, Biao& Zhou, Peng& Wang, Guoquan& Gao, Huawu& Hua, Rupeng…[et al.]. Huang-Pu-Tong-Qiao Formula Ameliorates Tau Phosphorylation by Inhibiting the CaM-CaMKIV Pathway. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1158273
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1158273
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر