Cordyceps militaris Induces Immunogenic Cell Death and Enhances Antitumor Immunogenic Response in Breast Cancer
المؤلفون المشاركون
Lee, Ji-Young
Quan, Xingguo
Kwak, Beom Seok
Lee, Anbok
Kim, Tae Hyun
Park, SaeGwang
Park, Jin Hee
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-09-03
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Cordyceps militaris has been widely used as a traditional medicine in East Asia.
Its effects against breast cancer have been reported previously.
However, whether C.
militaris-induced breast cancer cell death is immunogenic remains unelucidated.
This study aimed to determine whether ethanolic extracts of C.
militaris (CM-EE) could induce immunogenic cell death (ICD) in breast cancer immunotherapy to improve the efficacy of immune checkpoint inhibitors.
Human and mouse breast cancer cells were treated with various concentrations of CM-EE for 72 h, and cytotoxicity was measured using the sulforhodamine B assay.
Flow cytometry was used to assess cell death with annexin V/7-AAD staining and measure the surface exposure of damage-associated molecular pattern (DAMP) molecules including calreticulin, HSP70, and HSP90.
Western blot for cleaved poly (ADP-ribose) polymerase (PARP) was used to confirm apoptotic cell death.
The immunogenicity of CM-EE-induced dead cells was evaluated using the CFSE dilution assay.
CM-EE reduced the viability of human (MCF7, MDA-MB-231, HS578T, and SKBR3) and mouse (4T1-neu-HA, TUBO-HA, and TUBO-P2J-HA) breast cancer cells.
The IC50 was 25–50 µg/ml in human breast cancer cells and 10–50 µg/ml in mouse breast cancer cells at 72 h.
CM-EE-treated breast cancer cells were positively stained by annexin V, cleaved PARP, and cleaved caspase 3/7 which were increased upon CM-EE treatment.
Surface exposure of DAMP molecules was increased in dose- and time-dependent manners.
The CFSE dilution assay revealed that dendritic cells fed with CM-EE-treated breast cancer cells successfully stimulated tumor-specific T cell proliferation without inhibiting DC function and T cell proliferation.
The expression of PD-L1 mRNA and protein level was increased in dose-dependent manners.
In addition, CM-EE also potentiated the cytotoxic activity of tumor-specific T cells.
CM-EE can induce immunogenic and apoptotic cell death in breast cancer cells, and it is a good candidate for cancer immunotherapy and may improve the efficacy of immune checkpoint inhibitors.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Quan, Xingguo& Kwak, Beom Seok& Lee, Ji-Young& Park, Jin Hee& Lee, Anbok& Kim, Tae Hyun…[et al.]. 2020. Cordyceps militaris Induces Immunogenic Cell Death and Enhances Antitumor Immunogenic Response in Breast Cancer. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1158317
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Quan, Xingguo…[et al.]. Cordyceps militaris Induces Immunogenic Cell Death and Enhances Antitumor Immunogenic Response in Breast Cancer. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1158317
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Quan, Xingguo& Kwak, Beom Seok& Lee, Ji-Young& Park, Jin Hee& Lee, Anbok& Kim, Tae Hyun…[et al.]. Cordyceps militaris Induces Immunogenic Cell Death and Enhances Antitumor Immunogenic Response in Breast Cancer. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1158317
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1158317
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر