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Surface Coatings Modulate the Differences in the Adhesion Forces of Eukaryotic and Prokaryotic Cells as Detected by Single Cell Force Microscopy
المؤلفون المشاركون
المصدر
International Journal of Biomaterials
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-04-01
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Single cell force microscopy was used to investigate the maximum detachment force (MDF) of primary neuronal mouse cells (PNCs), osteoblastic cells (MC3T3), and prokaryotic cells (Staphylococcus capitis subsp.
capitis) from different surfaces after contact times of 1 to 5 seconds.
Positively charged silicon nitride surfaces were coated with positively charged polyethyleneimine (PEI) or poly-D-lysine.
Laminin was used as the second coating.
PEI induced MDFs of the order of 5 to 20 nN, slightly higher than silicon nitride did.
Lower MDFs (1 to 5 nN) were detected on PEI/laminin with the lowest on PDL/laminin.
To abstract from the individual cell properties, such as size, and to obtain cell type-specific MDFs, the MDFs of each cell on the different coatings were normalized to the silicon nitride reference for the longest contact time.
The differences in MDF between prokaryotic and eukaryotic cells were generally of similar dimensions, except on PDL/laminin, which discriminated against the prokaryotic cells.
We explain the lower MDFs on laminin by the spatial prevention of the electrostatic cell adhesion to the underlying polymers.
However, PEI can form long flexible loops protruding from the surface-bound layer that may span the laminin layer and easily bind to cellular surfaces and the small prokaryotic cells.
This was reflected in increased MDFs after two-second contact times on silicon nitride, whereas the two-second values were already observed after one second on PEI or PEI/laminin.
We assume that the electrostatic charge interaction with the PEI loops is more important for the initial adhesion of the smaller prokaryotic cells than for eukaryotic cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wysotzki, Philipp& Gimsa, Jan. 2019. Surface Coatings Modulate the Differences in the Adhesion Forces of Eukaryotic and Prokaryotic Cells as Detected by Single Cell Force Microscopy. International Journal of Biomaterials،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1158407
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wysotzki, Philipp& Gimsa, Jan. Surface Coatings Modulate the Differences in the Adhesion Forces of Eukaryotic and Prokaryotic Cells as Detected by Single Cell Force Microscopy. International Journal of Biomaterials No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1158407
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wysotzki, Philipp& Gimsa, Jan. Surface Coatings Modulate the Differences in the Adhesion Forces of Eukaryotic and Prokaryotic Cells as Detected by Single Cell Force Microscopy. International Journal of Biomaterials. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1158407
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1158407
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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