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Physicobiochemical Characteristics and Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells (hBM-MSCs) in Biodegradable Porous Sponge Bovine Cartilage Scaffold
المؤلفون المشاركون
Utomo, Dwikora Novembri
Mahyudin, Ferdiansyah
Wardhana, Teddy Heri
Purwati, Purwati
Brahmana, Febrian
Gusti, Agrippina Waya Rahmaning
المصدر
International Journal of Biomaterials
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-01-20
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Tissue engineering had been believed to overcome the limitation of cartilage lesions treatment.
Nowadays the studies focus on using mesenchymal stem cells in scaffold.
A biodegradable porous sponge bovine cartilage scaffold is expected to have the physicobiochemical characterization to promote chondrogenic differentiation of hBM-MSCs.
Scaffold from bovine cartilage was printed in 5 mm diameter sponge, categorized into nondecellularized (SBCS) and decellularized (DSBCS).
Physical characteristics (pore diameter and interconnectivity) were done using a Scanning Electron Microscope (SEM).
Biodegradability assessment used Phosphate Buffered Saline in 15, 30, 60 minutes, 6, 24, 48, 72 hours, and 1, 2 weeks.
The swelling ratios were counted in 5, 10, 15, 30, 60, and 360 minutes.
Biochemical characteristics were obtained by enzyme-linked immunosorbent assay for type II collagen, aggrecan, and Transforming Growth Factors-β (TGF-β).
Data were statistically compared.
hBM-MSCs were seeded on both scaffolds.
Histological examination used hematoxylin-eosin taken at the 2nd and 4th weeks after seeding.
There was no significant difference (p=0.473; p=0.142) on mean porosity 90.07 ± 4.64% vs.
88.93 ± 4.18% and pore diameter 111.83 ± 14.23 μm vs.
105.29 ± 11.14 μm assessment between SBCS and DSBCS groups.
Scaffolds from both groups showed pore interconnectivity.
DSBCS group had faster biodegradability.
SBCS group sweals better.
SBCS group contains type II collagen, aggrecan, and TGF-β with mean values 380.78 ± 18.63 ng/ml, 30.71 ± 4.50 ng/ml, and 130.12 ± 7.73 ng/ml, respectively, while DSBCS contained type II collagen, aggrecan, and TGF-β with mean values 64.83 ± 13.54 ng/ml, 8.41 ± 2.38 ng/ml, and 16.39 ± 4.49 ng/ml, respectively.
The results were statistically different (p<0.001).
Chondrocytes were found within scaffold on the 2nd and 4th weeks.
Physicobiochemical characteristic of biodegradable sponge bovine cartilage scaffold promotes chondrogenic differentiation of hBM-MSCs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Utomo, Dwikora Novembri& Mahyudin, Ferdiansyah& Wardhana, Teddy Heri& Purwati, Purwati& Brahmana, Febrian& Gusti, Agrippina Waya Rahmaning. 2019. Physicobiochemical Characteristics and Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells (hBM-MSCs) in Biodegradable Porous Sponge Bovine Cartilage Scaffold. International Journal of Biomaterials،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1158430
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Utomo, Dwikora Novembri…[et al.]. Physicobiochemical Characteristics and Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells (hBM-MSCs) in Biodegradable Porous Sponge Bovine Cartilage Scaffold. International Journal of Biomaterials No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1158430
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Utomo, Dwikora Novembri& Mahyudin, Ferdiansyah& Wardhana, Teddy Heri& Purwati, Purwati& Brahmana, Febrian& Gusti, Agrippina Waya Rahmaning. Physicobiochemical Characteristics and Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells (hBM-MSCs) in Biodegradable Porous Sponge Bovine Cartilage Scaffold. International Journal of Biomaterials. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1158430
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1158430
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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