Bushen Tiaojing (II and III) Decoctions Activate MAPK14 and MAPK31 to Promote the Expression of Cumulus Expansion-Related Factors in Mice
المؤلفون المشاركون
Liang, Xiao
Tong, Xue
Du, Hui-lan
He, Ming
Zhang, Yu
Duan, Yan-cang
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-02-18
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Background.
Bushen Tiaojing Decoctions (BSTJ-II-D and BSTJ-III-D) are used to assist pregnancy in clinical practice.
In this study, we explored the ability of sequential administration of BSTJ-II-D and BSTJ-III-D to promote cumulus cell (CC) expansion and its underlying mechanisms in controlled ovarian hyperstimulation (COH) mice.
Methods.
Kunming mice were randomly divided into three groups.
The normal group was injected intraperitoneally with saline, and distilled water was administered orally by gavage.
As the COH model, mice were injected with GnRHa, eCG, and hCG.
Subsequently, the BSTJD group received BSTJ-II-D and BSTJ-III-D orally by gavage, while the control group received distilled water.
We evaluated CC expansion and oocyte first polar body (PB1) extrusion under a stereomicroscope.
Serum levels of follicle-stimulating hormone (FSH) were detected by radioimmunoassay.
The expression of the CC expansion-related factors PTX3 and PTGS2 was detected by immunofluorescence, western blot, and quantitative real-time-polymerase chain reaction analyses (qRT-PCR).
Expression of p-MAPK14, p-MAPK3/1, MAPK14, and MAPK3/1 was detected by western blot analysis.
Results.
Sequential administration of BSTJ-II-D and BSTJ-III-D promoted cumulus expansion and oocyte PB1 extrusion and upregulated PTX3 and PTGS2 expression at the mRNA and protein levels.
Furthermore, the levels of p-MAPK14/MAPK14, p-MAPK3/1/MAPK3/1 proteins, and serum FSH in the BSTJD group were higher than those in the normal and control groups.
Conclusions.
Sequential administration of BSTJ-II-D and BSTJ-III-D promotes cumulus expansion and oocyte maturation in COH mice by increasing FSH expression and activating the MAPK14 and MAPK3/1 signalling pathways, thereby increasing expression of PTX3 and PTGS2.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liang, Xiao& Tong, Xue& Du, Hui-lan& He, Ming& Zhang, Yu& Duan, Yan-cang. 2020. Bushen Tiaojing (II and III) Decoctions Activate MAPK14 and MAPK31 to Promote the Expression of Cumulus Expansion-Related Factors in Mice. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1158445
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liang, Xiao…[et al.]. Bushen Tiaojing (II and III) Decoctions Activate MAPK14 and MAPK31 to Promote the Expression of Cumulus Expansion-Related Factors in Mice. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1158445
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liang, Xiao& Tong, Xue& Du, Hui-lan& He, Ming& Zhang, Yu& Duan, Yan-cang. Bushen Tiaojing (II and III) Decoctions Activate MAPK14 and MAPK31 to Promote the Expression of Cumulus Expansion-Related Factors in Mice. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1158445
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1158445
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر