Integrating Pharmacology and Microbial Network Analysis with Experimental Validation to Reveal the Mechanism of Composite Sophora Colon-Soluble Capsule against Ulcerative Colitis
المؤلفون المشاركون
Feng, Yang
Tong, Zhanqi
Gao, Lu
Chen, Mingjun
Ding, Yuxuan
Wang, Qiying
Wang, Shida
Song, Zitian
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-04-20
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
Ulcerative colitis (UC) has multifactorial pathogenesis that acts synergistically, such as immune system dysregulation and expansion of infectious gut microbiota.
Therefore, a multicomponent treatment derived from Chinese herbal medicine that interacts with multiple targets synergistically is needed.
Composite sophora colon-soluble capsule (CSCC) is a Chinese herbal formula that has shown therapeutic efficacy against UC in randomized clinical trials.
However, its bioactive components and potential target genes against UC remain unclear.
Here, we used a network pharmacology approach to detect component-target-pathway interactions of CSCC against UC.
A total of 29 gene targets, 91 bioactive components, and 20 enriched pathways of CSCC were identified.
The IL-17 signaling pathway activated by infectious gastrointestinal microbes and predicted by the network analysis to be a major pathway modulated by CSCC against UC was studied in a dextran sulfate sodium-induced colitis model.
CSCC showed remarkable efficacy against UC with respect to the attenuation of colon length, body weight loss, and disease activity index through gut microbiota recovery and intestinal immune homeostasis.
The rectal administration of CSCC reduced the numbers of Th17 cells isolated from both mesenteric lymph nodes and lamina propria mononuclear cells and the levels of IL-17A, IL-6, IL-1β, and TNF-α.
Additionally, the percentage of Treg cells and the levels of their hallmark cytokines were upregulated.
Rectal administration of CSCC led to microbiota regulation with a significant correlation between suppression of Verrucomicrobiaceae and Ruminococcaceae, as well as the elevation of Lactobacillaceae, and CSCC administration via microbiome correlation heatmaps and cooccurrence network analysis at multiple time points.
Thus, our study presents an effective herbal formula, CSCC, for UC treatment and explores its components and mechanisms of efficacy through the examination of gut microbiota and hallmark cytokines in the IL-17 pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ding, Yuxuan& Chen, Mingjun& Wang, Qiying& Gao, Lu& Feng, Yang& Wang, Shida…[et al.]. 2020. Integrating Pharmacology and Microbial Network Analysis with Experimental Validation to Reveal the Mechanism of Composite Sophora Colon-Soluble Capsule against Ulcerative Colitis. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1158562
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ding, Yuxuan…[et al.]. Integrating Pharmacology and Microbial Network Analysis with Experimental Validation to Reveal the Mechanism of Composite Sophora Colon-Soluble Capsule against Ulcerative Colitis. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-16.
https://search.emarefa.net/detail/BIM-1158562
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ding, Yuxuan& Chen, Mingjun& Wang, Qiying& Gao, Lu& Feng, Yang& Wang, Shida…[et al.]. Integrating Pharmacology and Microbial Network Analysis with Experimental Validation to Reveal the Mechanism of Composite Sophora Colon-Soluble Capsule against Ulcerative Colitis. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1158562
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1158562
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر