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Oridonin Attenuates Lipopolysaccharide-Induced ROS Accumulation and Inflammation in HK-2 Cells
المؤلفون المشاركون
Tzeng, I-Shiang
Hsieh, Po-Chun
Kuo, Chan-Yen
Lan, Chou-Chin
Huang, Jen-Hsuan
Hsu, Ya-Ting
Tsai, Cheng-Lin
Wang, Po
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-02-27
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Renal tubulointerstitial inflammation plays an important role in chronic kidney disease (CKD).
Inflammation reduction is a good strategy to combat CKD.
Oridonin, an ent-kaurane diterpenoid isolated from Rabdosia rubescens (Donglingcao), is considered as an effective natural candidate for the treatment of anti-inflammatory, antiviral, and antibacterial activities, including liver fibrosis and many tumors; however, no study has demonstrated its effect on lipopolysaccharide- (LPS-) induced renal inflammation.
To investigate the anti-inflammatory effects of oridonin on human renal proximal tubular epithelial cells (HK-2 cells), the expression levels of c-Jun N-terminal kinase (JNK) and reactive oxygen species (ROS) were evaluated by Western blot analysis and 2′,7′-dichlorofluorescein diacetate (DCF-DA) staining, respectively.
The level of intracellular ROS increased in a dose-dependent manner following LPS treatment, whereas oridonin inhibited this effect, suggestive of its ability to prevent ROS accumulation.
As the mitogen-activated protein kinase (MAPK) family of enzymes plays an important role in physiological responses, we examined the activation of JNK by Western blotting and found that oridonin attenuated LPS-induced JNK phosphorylation.
Oridonin also attenuated RAW 264.7 cell chemotaxis towards LPS-treated HK-2 cells.
Taken together, oridonin protected against LPS-induced inflammation including ROS accumulation, JNK activation, NF-κB nuclear translocation in HK-2 cells, and functionally blocked macrophage chemotaxis towards LPS-treated HK-2 cells.
Oridonin may exhibit therapeutic potential by the anti-inflammation effect in LPS-treated HK-2 cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Huang, Jen-Hsuan& Lan, Chou-Chin& Hsu, Ya-Ting& Tsai, Cheng-Lin& Tzeng, I-Shiang& Wang, Po…[et al.]. 2020. Oridonin Attenuates Lipopolysaccharide-Induced ROS Accumulation and Inflammation in HK-2 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1158680
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Huang, Jen-Hsuan…[et al.]. Oridonin Attenuates Lipopolysaccharide-Induced ROS Accumulation and Inflammation in HK-2 Cells. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1158680
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Huang, Jen-Hsuan& Lan, Chou-Chin& Hsu, Ya-Ting& Tsai, Cheng-Lin& Tzeng, I-Shiang& Wang, Po…[et al.]. Oridonin Attenuates Lipopolysaccharide-Induced ROS Accumulation and Inflammation in HK-2 Cells. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1158680
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1158680
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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