Synergistic Effect between Usnic Acid and Polymyxin B against Resistant Clinical Isolates of Pseudomonas aeruginosa
المؤلفون المشاركون
Vieira Maciel, Maria Amélia
Cavalcanti, Isabella Macário Ferro
Costa-Junior, Sérgio Dias
da Silva, Wagner Roberto Cirilo
da Silva, Adriana Maria Costa Marques
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-12
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The present study aimed to characterize the susceptibility profile of Pseudomonas aeruginosa and Acinetobacter spp.
clinical isolates to polymyxin B in a public hospital in Recife-PE, Brazil, between the years of 2018 and 2019, as well as to search for the presence of the mcr-1 gene and evaluate the interaction between polymyxin B and usnic acid against these isolates.
The strains were identified using the BD Phoenix™ automated system and the agar-spot test was used to determine the susceptibility profile to polymyxin B.
The minimum inhibitory concentrations (MICs) of usnic acid and polymyxin B were determined through the broth microdilution method according to the Clinical and Laboratory Standards Institute (CLSI).
Subsequently, Polymerase Chain Reaction (PCR) was performed to detect the mcr-1 gene in the isolates.
The interaction between usnic acid and polymyxin B was evaluated by the Checkerboard assay.
Among 34 isolates of P.
aeruginosa, 26.5% (9/34) were positive for the polymyxin B agar-spot test, and 11.8% (4/34) presented an intermediate susceptibility (MIC = 4 μg/mL), while 14.7% (5/34) presented antimicrobial resistance with MIC values ranging from 8 to 32 μg/mL.
Among 38 isolates of Acinetobacter spp., 13.2% (5/38) were positive for the polymyxin B agar-spot test and all of them were resistant to polymyxin B with a MIC value > 32 μg/mL.
The mcr-1 gene was not detected in the clinical isolates.
Regarding usnic acid, it presented a moderate antibacterial activity against two P.
aeruginosa isolates (MIC = 250 μg/mL) and no activity was detected against the others.
A synergistic effect between usnic acid and polymyxin B was observed against three clinical isolates of P.
aeruginosa which were resistant to polymyxin B (FICI ≤ 0.5).
Therefore, it was possible to observe that usnic acid is a promising candidate to be used in combination with polymyxin B against infections caused by resistant P.
aeruginosa.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Costa-Junior, Sérgio Dias& da Silva, Wagner Roberto Cirilo& da Silva, Adriana Maria Costa Marques& Vieira Maciel, Maria Amélia& Cavalcanti, Isabella Macário Ferro. 2020. Synergistic Effect between Usnic Acid and Polymyxin B against Resistant Clinical Isolates of Pseudomonas aeruginosa. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1158775
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Costa-Junior, Sérgio Dias…[et al.]. Synergistic Effect between Usnic Acid and Polymyxin B against Resistant Clinical Isolates of Pseudomonas aeruginosa. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1158775
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Costa-Junior, Sérgio Dias& da Silva, Wagner Roberto Cirilo& da Silva, Adriana Maria Costa Marques& Vieira Maciel, Maria Amélia& Cavalcanti, Isabella Macário Ferro. Synergistic Effect between Usnic Acid and Polymyxin B against Resistant Clinical Isolates of Pseudomonas aeruginosa. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1158775
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1158775
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر