In Vitro Evaluation of Proliferation and Migration Behaviour of Human Bone Marrow-Derived Mesenchymal Stem Cells in Presence of Platelet-Rich Plasma
المؤلفون المشاركون
Pham, Thuy Anh Vu
Nguyen, Anh Thi Mai
Tran, Ha Le Bao
المصدر
International Journal of Dentistry
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-04-09
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Objective.
To access the effects of platelet-rich plasma (PRP) on the behaviour of human bone marrow-derived mesenchymal stem cells (hBMSCs), including proliferation and migration.
Methods.
PRP was diluted with DMEM/F12, resulting in concentrations of 1%, 2%, and 5%.
The proliferation of hBMSCs was examined by 2 methods: cell-number counting with the haemocytometer method and the colony-forming unit-fibroblast (CFU-F) assay.
Cell migration was evaluated using the scratch wound healing (SWH) assay; after that, the recorded digital images were analysed by the Image-Analysis J 1.51j8 software to compare the cell-free areas between groups after 0, 24, and 48 hours.
Results.
hBMSCs cultured in DMEM/F12 at PRP concentrations of 1%, 2%, and 5% were all able to proliferate and migrate.
In the 5% PRP group, hBMSCs proliferated greatly with a significantly higher cell number than reported for all other groups on days 5, 7, and 9.
CFU-Fs were observed in all groups, except for the negative control group.
The SWH assay demonstrated that hBMSCs cultured in 2% and 5% PRP almost filled the artificial wound scratch and significantly migrated more than those of all other groups at both 24 h and 48 h.
Conclusion.
This study indicated that, due to the significant enhancement of cell proliferation and migration, 5% PRP might be the optimal concentration that should be used to promote the potential of hBMSCs in wound healing.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nguyen, Anh Thi Mai& Tran, Ha Le Bao& Pham, Thuy Anh Vu. 2019. In Vitro Evaluation of Proliferation and Migration Behaviour of Human Bone Marrow-Derived Mesenchymal Stem Cells in Presence of Platelet-Rich Plasma. International Journal of Dentistry،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1159144
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nguyen, Anh Thi Mai…[et al.]. In Vitro Evaluation of Proliferation and Migration Behaviour of Human Bone Marrow-Derived Mesenchymal Stem Cells in Presence of Platelet-Rich Plasma. International Journal of Dentistry No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1159144
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nguyen, Anh Thi Mai& Tran, Ha Le Bao& Pham, Thuy Anh Vu. In Vitro Evaluation of Proliferation and Migration Behaviour of Human Bone Marrow-Derived Mesenchymal Stem Cells in Presence of Platelet-Rich Plasma. International Journal of Dentistry. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1159144
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1159144
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر