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Comparative Proteomic Analysis of Visceral Adipose Tissue in Morbidly Obese and Normal Weight Chinese Women
المؤلفون المشاركون
Gong, Fengying
Zhu, Hui juan
Pan, Hui
Wang, Lin jie
Yang, Hong bo
Sun, Wei
Shang, Chen
Wang, Chunlin
Wang, Xiangqing
Wang, Xue
المصدر
International Journal of Endocrinology
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-18
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Objective.
Visceral adipose tissue (VAT) plays a central role in the balance of energy metabolism.
The objective of this study was to investigate the differentially expressed proteins in VAT between morbidly obese (BMI >35 kg/m2) and normal weight Chinese women.
Method.
Nine morbidly obese women and 8 normal weight women as controls were enrolled.
Abdominal VAT was excised and analyzed by label-free one-dimensional liquid chromatography tandem mass spectrometry (1D-LC-MS/MS).
Differentially expressed VAT proteins were further analyzed with Gene Ontology (GO) analysis and Ingenuity Pathway Analysis (IPA).
Masson’s trichrome staining and CD68 immunohistochemical staining of VAT were conducted in all subjects.
Result.
A total of 124 differentially expressed proteins were found with a ≥2-fold difference.
Forty-one proteins were upregulated, and 83 proteins were downregulated in obese individuals.
These altered VAT proteins were involved in the attenuation of the liver X receptor/retinoid X receptor (LXR/RXR) signaling pathway and the activation of the acute-phase response process.
Three proteins (ACSL1, HADH, and UCHL1) were validated by western blotting using the same set of VAT samples from 6 morbidly obese and 7 normal weight patients, and the results indicated that the magnitude and direction of the protein changes were in accordance with the proteomic analysis.
Masson’s trichrome staining and CD68 immunohistochemical staining demonstrated that there was much more collagen fiber deposition and CD68-positive macrophages in the VAT of morbidly obese patients, suggesting extensive fiber deposition and macrophage infiltration.
Conclusion.
A number of differentially expressed proteins were identified in VAT between morbidly obese and normal weight Chinese females.
These differential proteins could be potential candidates in addressing the role of VAT in the development of obesity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shang, Chen& Sun, Wei& Wang, Chunlin& Wang, Xiangqing& Zhu, Hui juan& Wang, Lin jie…[et al.]. 2019. Comparative Proteomic Analysis of Visceral Adipose Tissue in Morbidly Obese and Normal Weight Chinese Women. International Journal of Endocrinology،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1159378
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shang, Chen…[et al.]. Comparative Proteomic Analysis of Visceral Adipose Tissue in Morbidly Obese and Normal Weight Chinese Women. International Journal of Endocrinology No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1159378
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shang, Chen& Sun, Wei& Wang, Chunlin& Wang, Xiangqing& Zhu, Hui juan& Wang, Lin jie…[et al.]. Comparative Proteomic Analysis of Visceral Adipose Tissue in Morbidly Obese and Normal Weight Chinese Women. International Journal of Endocrinology. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1159378
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1159378
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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