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A Possible Mechanism of Metformin in Improving Insulin Resistance in Diabetic Rat Models
المؤلفون المشاركون
Pang, Shuguang
Li, Mengsiyu
Hu, Xiaowen
Xu, Yeqiu
Hu, Xiaolin
Zhang, Chunxue
المصدر
International Journal of Endocrinology
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-10-13
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Background.
Type 2 diabetes has become one of the most common diseases worldwide, causing a serious social burden.
As a first-line treatment for diabetes, metformin can effectively improve insulin resistance.
It has been reported that 12α-hydroxylated BA (mainly CA) is associated with insulin resistance.
The purpose of this study was to analyze the changes in CA and possible signaling mechanisms in diabetic rats after metformin intervention.
Methods.
HepG2 cells were cultured after adding different concentrations of metformin.
The cell viability was measured using CCK8 kit, and the expression of FXR, MAFG, and CYP8B1 in cells was detected by WB.
The rat models of type 2 diabetes were induced by low-dose streptozotocin by feeding a high-fat diet, and the control rats (CON) were fed on normal food; the diabetic rats (DM) were given a high-fat diet without supplementation with metformin, while the metformin-treated diabetic rats (DM + MET) were given a high-fat diet and supplemented with metformin.
Biochemical parameters were detected at the end of the test.
Expression levels of FXR, CYP8B1, and MAFG were assessed by WB.
Serum CA were measured using an enzyme-linked immunosorbent assay (ELISA).
Results.
In HepG2 cells, metformin dose-dependently enhanced the transcriptional activity of FXR and MAFG and inhibited the expression of CYP8B1.
Metformin-treated DM rats showed improved glucose and bile acid metabolism.
In addition, significantly increased FXR and MAFG and decreased CYP8B1 were observed in DM + MET rats.
At the same time, the CA content of metformin-treated rats was lower than that of diabetic rats.
Conclusion.
Changes in CA synthesis after metformin treatment may be associated with inhibition of CYP8B1.
These results may play an important role in improving insulin sensitivity after metformin treatment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Mengsiyu& Hu, Xiaowen& Xu, Yeqiu& Hu, Xiaolin& Zhang, Chunxue& Pang, Shuguang. 2019. A Possible Mechanism of Metformin in Improving Insulin Resistance in Diabetic Rat Models. International Journal of Endocrinology،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1159459
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Mengsiyu…[et al.]. A Possible Mechanism of Metformin in Improving Insulin Resistance in Diabetic Rat Models. International Journal of Endocrinology No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1159459
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Mengsiyu& Hu, Xiaowen& Xu, Yeqiu& Hu, Xiaolin& Zhang, Chunxue& Pang, Shuguang. A Possible Mechanism of Metformin in Improving Insulin Resistance in Diabetic Rat Models. International Journal of Endocrinology. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1159459
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1159459
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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