miR-29a Negatively Affects Glucose-Stimulated Insulin Secretion and MIN6 Cell Proliferation via Cdc42β-Catenin Signaling
المؤلفون المشاركون
Duan, Jing
Qian, Xian-Ling
Li, Jun
Xiao, Xing-Hua
Lu, Xiang-Tong
Lv, Lin-Chen
Huang, Qing-Yun
Ding, Wen
Zhang, Hong-Yan
Xiong, Li-Xia
المصدر
International Journal of Endocrinology
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-08-28
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Background.
Diabetes is a progressive metabolic disease characterized by hyperglycemia.
Functional impairment of islet β cells can occur to varying degrees.
This impairment can initially be compensated for by proliferation and metabolic changes of β cells.
Cell division control protein 42 (Cdc42) and the microRNA (miRNA) miR-29 have important roles in β-cell proliferation and glucose-stimulated insulin secretion (GSIS), which we further explored using the mouse insulinoma cell line MIN6.
Methods.
Upregulation and downregulation of miR-29a and Cdc42 were accomplished using transient transfection.
miR-29a and Cdc42 expression was detected by real-time PCR and western blotting.
MIN6 proliferation was detected using a cell counting kit assay.
GSIS under high-glucose (20.0 mM) or basal-glucose (5.0 mM) stimulation was detected by enzyme-linked immunosorbent assay.
The miR-29a binding site in the Cdc42 mRNA 3′-untranslated region (UTR) was determined using bioinformatics and luciferase reporter assays.
Results.
miR-29a overexpression inhibited proliferation (P<0.01) and GSIS under high-glucose stimulation (P<0.01).
Cdc42 overexpression promoted proliferation (P<0.05) and GSIS under high-glucose stimulation (P<0.05).
miR-29a overexpression decreased Cdc42 expression (P<0.01), whereas miR-29a downregulation increased Cdc42 expression (P<0.01).
The results showed that the Cdc42 mRNA 3′-UTR is a direct target of miR-29a in vitro.
Additionally, Cdc42 reversed miR-29a-mediated inhibition of proliferation and GSIS (P<0.01).
Furthermore, miR-29a inhibited β-catenin expression (P<0.01), whereas Cdc42 promoted β-catenin expression (P<0.01).
Conclusion.
By negatively regulating Cdc42 and the downstream molecule β-catenin, miR-29a inhibits MIN6 proliferation and insulin secretion.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Duan, Jing& Qian, Xian-Ling& Li, Jun& Xiao, Xing-Hua& Lu, Xiang-Tong& Lv, Lin-Chen…[et al.]. 2019. miR-29a Negatively Affects Glucose-Stimulated Insulin Secretion and MIN6 Cell Proliferation via Cdc42β-Catenin Signaling. International Journal of Endocrinology،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1159590
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Duan, Jing…[et al.]. miR-29a Negatively Affects Glucose-Stimulated Insulin Secretion and MIN6 Cell Proliferation via Cdc42β-Catenin Signaling. International Journal of Endocrinology No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1159590
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Duan, Jing& Qian, Xian-Ling& Li, Jun& Xiao, Xing-Hua& Lu, Xiang-Tong& Lv, Lin-Chen…[et al.]. miR-29a Negatively Affects Glucose-Stimulated Insulin Secretion and MIN6 Cell Proliferation via Cdc42β-Catenin Signaling. International Journal of Endocrinology. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1159590
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1159590
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر