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Ginsenoside Rg1 Ameliorates Palmitic Acid-Induced Hepatic Steatosis and Inflammation in HepG2 Cells via the AMPKNF-κB Pathway
المؤلفون المشاركون
Zhao, Jinqiu
Xiao, Qing
Zhang, Shujun
Yang, Cheng
Du, Ruoyang
Li, Jiajun
Xu, Yashu
Qin, Yuanyuan
Gao, Yue
Huang, Wenxiang
المصدر
International Journal of Endocrinology
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-07-28
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Nonalcoholic fatty liver disease (NAFLD) is one of the common diseases in the world, and it can progress from simple lipid accumulation to sustained inflammation.
The present study was designed to investigate the effects and underlying mechanisms of ginsenoside Rg1 (G-Rg1) treatment on NAFLD in vitro.
HepG2 cells were treated with palmitic acid (PA) to induce steatosis and inflammation and then successively incubated with G-Rg1.
Lipids accumulation was analyzed by Oil Red O staining and intracellular triglyceride (TG) quantification.
Inflammatory conditions were examined by quantifying the levels of cell supernatant alanine transaminase/aspartate aminotransferase (ALT/AST) and secretory proinflammatory cytokines, including IL-1β, IL-6, and TNF-α in the cell supernatants.
Quantitative RT-PCR and western blotting were used to measure the expressions of genes and proteins associated with lipogenic synthesis and inflammation, including AMP-activated protein kinase (AMPK) and nuclear factor-kappa B (NF-κB) pathways.
HepG2 cells were pretreated with an AMPK inhibitor; then, Oil Red O staining and TG quantification were performed to study the lipid deposition.
Phospho-AMPK (Thr172) (p-AMPK) and phospho-acetyl-CoA carboxylase (Ser79) (p-ACCα) were quantified by immunoblotting.
Immunofluorescence was performed to demonstrate the nuclear translocation of NF-κB P65.
The present study showed that PA markedly increased the intracellular lipid droplets accumulation and TG levels, but decreased AMPK phosphorylation and the expressions of its downstream lipogenic genes.
However, G-Rg1 alleviated hepatic steatosis and reduced the intracellular TG content; these changes were accompanied by the activation of the AMPK pathway.
In addition, blocking AMPK by using the AMPK inhibitor markedly abolished the G-Rg1-mediated protection against PA-induced lipid deposition in HepG2 cells.
Furthermore, G-Rg1 reduced the ALT/AST levels and proinflammatory cytokines release, which were all enhanced by PA.
These effects were correlated with the inactivation of the NF-κB pathway and translocation of P65 from the cytoplasm to the nucleus.
Overall, these results suggest that G-Rg1 effectively ameliorates hepatic steatosis and inflammation, which might be associated with the AMPK/NF-κB pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xiao, Qing& Zhang, Shujun& Yang, Cheng& Du, Ruoyang& Zhao, Jinqiu& Li, Jiajun…[et al.]. 2019. Ginsenoside Rg1 Ameliorates Palmitic Acid-Induced Hepatic Steatosis and Inflammation in HepG2 Cells via the AMPKNF-κB Pathway. International Journal of Endocrinology،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1159712
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xiao, Qing…[et al.]. Ginsenoside Rg1 Ameliorates Palmitic Acid-Induced Hepatic Steatosis and Inflammation in HepG2 Cells via the AMPKNF-κB Pathway. International Journal of Endocrinology No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1159712
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xiao, Qing& Zhang, Shujun& Yang, Cheng& Du, Ruoyang& Zhao, Jinqiu& Li, Jiajun…[et al.]. Ginsenoside Rg1 Ameliorates Palmitic Acid-Induced Hepatic Steatosis and Inflammation in HepG2 Cells via the AMPKNF-κB Pathway. International Journal of Endocrinology. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1159712
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1159712
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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