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Silicone Substrate with Collagen and Carbon Nanotubes Exposed to Pulsed Current for MSC Osteodifferentiation
المؤلفون المشاركون
Jamal, Daniyal
de Guzman, Roche C.
المصدر
International Journal of Biomaterials
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-08-23
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Autologous human adipose tissue-derived mesenchymal stem cells (MSCs) have the potential for clinical translation through their induction into osteoblasts for regeneration.
Bone healing can be driven by biophysical stimulation using electricity for activating quiescent adult stem cells.
It is hypothesized that application of electric current will enhance their osteogenic differentiation, and addition of conductive carbon nanotubes (CNTs) to the cell substrate will provide increased efficiency in current transmission.
Cultured MSCs were seeded and grown onto fabricated silicone-based composites containing collagen and CNT fibers.
Chemical inducers, namely, glycerol phosphate, dexamethasone, and vitamin C, were then added to the medium, and pulsatile submilliampere electrical currents (about half mA for 5 cycles at 4 mHz, twice a week) were applied for two weeks.
Calcium deposition indicative of MSC differentiation and osteoblastic activity was quantified through Alizarin Red S and spectroscopy.
It was found that pulsed current significantly increased osteodifferentiation on silicone-collagen films without CNTs.
Under no external current, the presence of 10% (m/m) CNTs led to a significant and almost triple upregulation of calcium deposition.
Both CNTs and current parameters did not appear to be synergistic.
These conditions of enhanced osteoblastic activities may further be explored ultimately towards future therapeutic use of MSCs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jamal, Daniyal& de Guzman, Roche C.. 2017. Silicone Substrate with Collagen and Carbon Nanotubes Exposed to Pulsed Current for MSC Osteodifferentiation. International Journal of Biomaterials،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1160075
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jamal, Daniyal& de Guzman, Roche C.. Silicone Substrate with Collagen and Carbon Nanotubes Exposed to Pulsed Current for MSC Osteodifferentiation. International Journal of Biomaterials No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1160075
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jamal, Daniyal& de Guzman, Roche C.. Silicone Substrate with Collagen and Carbon Nanotubes Exposed to Pulsed Current for MSC Osteodifferentiation. International Journal of Biomaterials. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1160075
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1160075
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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