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Thrombopoietin Secretion by Human Ovarian Cancer Cells
المؤلفون المشاركون
Besbes, Samaher
Shah, Shahid
Al-dybiat, Iman
Mirshahi, Shahsoltan
Helfer, Helene
Najah, Haythem
Fourgeaud, Caroline
Ghedira, Ibtissem
Soria, Jeannette
Mirshahi, Massoud
Pocard, Marc
المصدر
International Journal of Cell Biology
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-03-30
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The thrombopoietin (TPO) gene expression in human ovary and cancer cells from patients with ovarian carcinomatosis, as well as several cancer cell lines including MDA-MB231 (breast cancer), K562 and HL60 (Leukemic cells), OVCAR-3NIH and SKOV-3 (ovarian cancer), was performed using RT PCR, real-time PCR, and gene sequencing.
Human liver tissues are used as controls.
The presence of TPO in the cells and its regulation by activated protein C were explored by flow cytometry.
TPO content of cell extract as well as plasma of a patient with ovarian cancer was evaluated by ELISA.
The functionality of TPO was performed in coculture on the basis of the viability of a TPO-dependent cell line (Ba/F3), MTT assay, and Annexin-V labeling.
As in liver, ovarian tissues and all cancer cells lines except the MDA-MB231 express the three TPO-1 (full length TPO), TPO-2 (12 bp deletion), and TPO-3 (116 pb deletion) variants.
Primary ovarian cancer cells as well as cancer cell lines produce TPO.
The thrombopoietin production by OVCAR-3 increased when cells are stimulated by aPC.
OVCAR-3 cell’s supernatant can replace exogenous TPO and inhibited TPO-dependent cell line (Ba/F3) apoptosis.
The thrombopoietin produced by tumor may have a direct effect on thrombocytosis/thrombosis occurrence in patients with ovarian cancer.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Besbes, Samaher& Shah, Shahid& Al-dybiat, Iman& Mirshahi, Shahsoltan& Helfer, Helene& Najah, Haythem…[et al.]. 2017. Thrombopoietin Secretion by Human Ovarian Cancer Cells. International Journal of Cell Biology،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1165126
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Besbes, Samaher…[et al.]. Thrombopoietin Secretion by Human Ovarian Cancer Cells. International Journal of Cell Biology No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1165126
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Besbes, Samaher& Shah, Shahid& Al-dybiat, Iman& Mirshahi, Shahsoltan& Helfer, Helene& Najah, Haythem…[et al.]. Thrombopoietin Secretion by Human Ovarian Cancer Cells. International Journal of Cell Biology. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1165126
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1165126
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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