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Thrombin-Induced Calpain Activation Promotes Protease-Activated Receptor 1 Internalization
المؤلفون المشاركون
Alvarez-Arce, Alejandro
Lee-Rivera, Irene
López, Edith
Hernández-Cruz, Arturo
López-Colomé, Ana María
المصدر
International Journal of Cell Biology
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-11-09
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
The serine protease thrombin activates Protease-Activated Receptors (PARs), a family of G-protein-coupled receptors (GPCRs) activated by the proteolytic cleavage of their extracellular N-terminal domain.
Four members of this family have been identified: PAR1–4.
The activation of Protease-Activated Receptor 1(PAR1), the prototype of this receptor family, leads to an increase in intracellular Ca+2 concentration ([Ca+2]i) mediated by Gq11α coupling and phospholipase C (PLC) activation.
We have previously shown that the stimulation of PAR1 by thrombin promotes intracellular signaling leading to RPE cell transformation, proliferation, and migration which characterize fibroproliferative eye diseases leading to blindness.
Within this context, the elucidation of the mechanisms involved in PAR1 inactivation is of utmost importance.
Due to the irreversible nature of PAR1 activation, its inactivation must be efficiently regulated in order to terminate signaling.
Using ARPE-19 human RPE cell line, we characterized thrombin-induced [Ca+2]i increase and demonstrated the calcium-dependent activation of μ-calpain mediated by PAR1.
Calpains are a family of calcium-activated cysteine proteases involved in multiple cellular processes including the internalization of membrane proteins through clathrin-coated vesicles.
We demonstrated that PAR1-induced calpain activation results in the degradation of α-spectrin by calpain, essential for receptor endocytosis, and the consequent decrease in PAR1 membrane expression.
Collectively, the present results identify a novel μ-calpain-dependent mechanism for PAR1 inactivation following exposure to thrombin.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Alvarez-Arce, Alejandro& Lee-Rivera, Irene& López, Edith& Hernández-Cruz, Arturo& López-Colomé, Ana María. 2017. Thrombin-Induced Calpain Activation Promotes Protease-Activated Receptor 1 Internalization. International Journal of Cell Biology،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1165130
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Alvarez-Arce, Alejandro…[et al.]. Thrombin-Induced Calpain Activation Promotes Protease-Activated Receptor 1 Internalization. International Journal of Cell Biology No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1165130
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Alvarez-Arce, Alejandro& Lee-Rivera, Irene& López, Edith& Hernández-Cruz, Arturo& López-Colomé, Ana María. Thrombin-Induced Calpain Activation Promotes Protease-Activated Receptor 1 Internalization. International Journal of Cell Biology. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1165130
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1165130
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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