Genomic Analysis of a New Estrogen-Degrading Bacterial Strain, Acinetobacter sp. DSSKY-A-001
المؤلفون المشاركون
Qiu, Qing
Wang, Ping
Kang, Hui
Wang, Yu
Tian, Kejian
Huo, Hongliang
المصدر
International Journal of Genomics
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-06-02
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
In this study, we isolated a new estrogen-degrading bacterium from a soil sample collected near a pharmaceutical factory in Beijing, China.
Morphological observations, physiological and biochemical analyses, and sequence analysis showed that the strain was in the genus Acinetobacter, and it was named DSSKY-A-001.
The estrogen degradation rate and growth density of strain DSSKY-A-001 were determined by high-performance liquid chromatography and a growth assay using a microplate reader, respectively.
The estrogen degradation rate was 76% on the third day and 90% on the sixth day of culture.
Three kinds of estrogen metabolism intermediates were detected by high-performance liquid chromatography and mass spectrometry, and the estrogen metabolic pathway and possible estrogen-degrading enzymes were predicted.
RT-PCR was used to verify whether the three putative enzymes, catechol 1,2-dioxygenase, dioxygenase, and 7α-hydroxysteroid dehydrogenase, were expressed in the strain.
The results of the validation were consistent with the predictions that these three enzymes were present and expressed in Acinetobacter DSSKY-A-001.
To further understand the estrogen-degrading activity of the strain at the genetic level, we sequenced the genome and performed a functional gene annotation.
Through this gene sequence analysis, we identified genes predicted to encode the previously detected enzymes, catechol 1,2-dioxygenase, dioxygenase, and 7α-hydroxysteroid dehydrogenase, as well as six other enzymes that may be involved in estrogen degradation.
Therefore, a total of nine enzymes related to estrogen degradation were found.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Qiu, Qing& Wang, Ping& Kang, Hui& Wang, Yu& Tian, Kejian& Huo, Hongliang. 2019. Genomic Analysis of a New Estrogen-Degrading Bacterial Strain, Acinetobacter sp. DSSKY-A-001. International Journal of Genomics،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1165551
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Qiu, Qing…[et al.]. Genomic Analysis of a New Estrogen-Degrading Bacterial Strain, Acinetobacter sp. DSSKY-A-001. International Journal of Genomics No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1165551
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Qiu, Qing& Wang, Ping& Kang, Hui& Wang, Yu& Tian, Kejian& Huo, Hongliang. Genomic Analysis of a New Estrogen-Degrading Bacterial Strain, Acinetobacter sp. DSSKY-A-001. International Journal of Genomics. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1165551
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1165551
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر