RNA-Seq-Based Gene Expression Pattern and Morphological Alterations in Chick Thymus during Postnatal Development
المؤلفون المشاركون
Zhong, Ju-Ming
Xue, Zhouyiyuan
Ansari, Abdur Rahman
Zhao, Xing
Zang, Kun
Liang, Yu
Cui, Lei
Hu, Yafang
Cheng, Ranran
Zhang, Xiaolong
Liu, Huazhen
المصدر
International Journal of Genomics
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-04-21
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
The thymus is a lobulated unique lymphoid immune organ that plays a critical role in the selection, development, proliferation, and differentiation of T cells.
The thymus of developing chickens undergoes continued morphological alterations; however, the biomolecular and transcriptional dynamics of the postnatal thymus in avian species is not clear yet.
Therefore, the thymuses from chickens at different stages of development (at weeks 0, 1, 5, 9, 18, and 27) were used in the present study.
The RNA-seq method was used to study the gene expression patterns.
On average, 24120819 clean reads were mapped, differentially expressed genes (DEGs) were identified on the basis of log values (fold change), including 744 upregulated and 425 downregulated genes.
The expression pattern revealed by RNA-seq was validated by quantitative real-time PCR (qPCR) analysis of four important genes, which are PCNA, CCNA2, CCNB2, and CDK1.
Thus, the current study revealed that during postnatal development, the thymus undergoes severe atrophy.
Thymus structure was damaged and gene expression changed dramatically, especially at the 27th week of age.
Moreover, we found significant changes of several signaling pathways such as the cytokine-cytokine receptor interaction and cell cycle signaling pathways.
Hence, it may be inferred that those signaling pathways might be closely related to the postnatal chicken thymus development.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xue, Zhouyiyuan& Ansari, Abdur Rahman& Zhao, Xing& Zang, Kun& Liang, Yu& Liu, Huazhen…[et al.]. 2019. RNA-Seq-Based Gene Expression Pattern and Morphological Alterations in Chick Thymus during Postnatal Development. International Journal of Genomics،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1165686
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Huazhen…[et al.]. RNA-Seq-Based Gene Expression Pattern and Morphological Alterations in Chick Thymus during Postnatal Development. International Journal of Genomics No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1165686
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xue, Zhouyiyuan& Ansari, Abdur Rahman& Zhao, Xing& Zang, Kun& Liang, Yu& Liu, Huazhen…[et al.]. RNA-Seq-Based Gene Expression Pattern and Morphological Alterations in Chick Thymus during Postnatal Development. International Journal of Genomics. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1165686
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1165686
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر