Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress
المؤلفون المشاركون
Zhao, Chen
Lin, Mengjuan
Pan, Yasi
Yu, Baoping
المصدر
Gastroenterology Research and Practice
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-04
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Background.
Visceral hypersensitivity is a common feature of irritable bowel syndrome.
Cholinergic system involves in the development of visceral hypersensitivity, and high-affinity choline transporter (CHT1) is of crucial importance in choline uptake system.
However, involvement of CHT1 in visceral hypersensitivity remains unknown.
The research aimed to study the CHT1 expression in dorsal root ganglions (DRGs) and the role of CHT1 in visceral hypersensitivity.
Methods.
Repetitive water avoidance stress (WAS) was used to induce visceral hypersensitivity in rats.
Colorectal distension (CRD) was determined, and the abdominal withdrawal reflex (AWR) and threshold intensity data were recorded to measure the visceral sensitivity.
After intraperitoneal injection of hemicholinium-3 (HC-3), the specific inhibitor of CHT1, CRD data were also recorded.
The CHT1 expression of DRGs was investigated by Western blotting, immunohistochemistry, and quantitative RT-PCR.
Acetylcholine levels in the DRGs were detected by the assay kit.
Results.
Repetitive WAS increased the AWR score of CRD at high distension pressure and decreased the mean threshold of rats.
The CHT1 expression and acetylcholine concentration of DRG were significantly increased in WAS rats.
After the administration of HC-3, the AWR score in WAS group was significantly increased at higher distension pressure while the threshold intensity was significantly reduced compared to the normal saline group.
Acetylcholine concentration was significantly lower than the normal saline rats.
Conclusion.
Our research firstly reports that CHT1 is overexpressed in noninflammatory visceral hypersensitivity, and blockage of CHT1 can enhance the visceral hypersensitivity.
CHT1 may play an inhibitory role in visceral hypersensitivity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhao, Chen& Lin, Mengjuan& Pan, Yasi& Yu, Baoping. 2018. Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress. Gastroenterology Research and Practice،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1165926
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhao, Chen…[et al.]. Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress. Gastroenterology Research and Practice No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1165926
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhao, Chen& Lin, Mengjuan& Pan, Yasi& Yu, Baoping. Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress. Gastroenterology Research and Practice. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1165926
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1165926
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر