A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal
المؤلفون المشاركون
Badiane, Aida Sadikh
Ndiaye, Mouhamadou Mansour
Diongue, Khadim
Seck, Mame Cheikh
Diallo, Mamadou Alpha
Ndiaye, Daouda
Bréchard, Ludivine
Ranque, Stéphane
المصدر
International Journal of Microbiology
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-19
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Classically, dermatophytes are identified by phenotypic methods even if these methods, sometimes, remain difficult or uncertain.
On the other hand, nucleotide sequence analysis of internal transcribed spacers (ITS) of rDNA has proved to be a useful method for identification of dermatophytes.
The objective of this study was to compare the phenotypic method with DNA sequencing of the ITS regions for identification of dermatophyte species isolated in Dakar, Senegal.
A collection of thirty-two strains of dermatophytes were isolated from patients suffering from dermatophytosis.
Mycological identification revealed Trichophyton soudanense (n = 13), T.
interdigitale (n = 10), Microsporum audouinii (n = 5), and one strain for each of the following species: T.
rubrum, T.
mentagrophytes, and M.
canis and one unidentified strain.
For comparison, ITS-based PCR and DNA sequencing were applied for identification of the isolated dermatophytes.
ITS sequences showed, in BLAST search analysis, 99-100% of similarity.
Identification of dermatophyte isolates by conventional methods was confirmed by DNA sequencing of the ITS regions in 84% of cases.
Discrepancies concern mostly T.
rubrum misidentified as T.
interdigitale.
PCR sequencing provided an excellent tool for identifying dermatophyte strains that do not present typical morphological characteristics.
It was also able to give correct identification of an atypical strain of M.
audouinii responsible of mycetoma of the scalp.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Diongue, Khadim& Bréchard, Ludivine& Diallo, Mamadou Alpha& Seck, Mame Cheikh& Ndiaye, Mouhamadou Mansour& Badiane, Aida Sadikh…[et al.]. 2019. A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal. International Journal of Microbiology،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1166275
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Diongue, Khadim…[et al.]. A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal. International Journal of Microbiology No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1166275
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Diongue, Khadim& Bréchard, Ludivine& Diallo, Mamadou Alpha& Seck, Mame Cheikh& Ndiaye, Mouhamadou Mansour& Badiane, Aida Sadikh…[et al.]. A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal. International Journal of Microbiology. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1166275
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1166275
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر