Development and Validation of a Simple High Performance Liquid ChromatographyUV Method for Simultaneous Determination of Urinary Uric Acid, Hypoxanthine, and Creatinine in Human Urine
المؤلفون المشاركون
Perera, Hemamali
Soysa, Preethi
Wijesundera, W. S. S.
Wijemanne, Nimanthi
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-05-15
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Uric acid and hypoxanthine are produced in the catabolism of purine.
Abnormal urinary levels of these products are associated with many diseases and therefore it is necessary to have a simple and rapid method to detect them.
Hence, we report a simple reverse phase high performance liquid chromatography (HPLC/UV) technique, developed and validated for simultaneous analysis of uric acid, hypoxanthine, and creatinine in human urine.
Urine was diluted appropriately and eluted with C-18 column 100 mm × 4.6 mm with a C-18 precolumn 25 mm × 4.6 mm in series.
Potassium phosphate buffer (20 mM, pH 7.25) at a flow rate of 0.40 mL/min was employed as the solvent and peaks were detected at 235 nm.
Tyrosine was used as the internal standard.
The experimental conditions offered a good separation of analytes without interference of endogenous substances.
The calibration curves were linear for all test compounds with a regression coefficient, r2>0.99.
Uric acid, creatinine, tyrosine, and hypoxanthine were eluted at 5.2, 6.1, 7.2, and 8.3 min, respectively.
Intraday and interday variability were less than 4.6% for all the analytes investigated and the recovery ranged from 98 to 102%.
The proposed HPLC procedure is a simple, rapid, and low cost method with high accuracy with minimum use of organic solvents.
This method was successfully applied for the determination of creatinine, hypoxanthine, and uric acid in human urine.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wijemanne, Nimanthi& Soysa, Preethi& Wijesundera, W. S. S.& Perera, Hemamali. 2018. Development and Validation of a Simple High Performance Liquid ChromatographyUV Method for Simultaneous Determination of Urinary Uric Acid, Hypoxanthine, and Creatinine in Human Urine. International Journal of Analytical Chemistry،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1166297
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wijemanne, Nimanthi…[et al.]. Development and Validation of a Simple High Performance Liquid ChromatographyUV Method for Simultaneous Determination of Urinary Uric Acid, Hypoxanthine, and Creatinine in Human Urine. International Journal of Analytical Chemistry No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1166297
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wijemanne, Nimanthi& Soysa, Preethi& Wijesundera, W. S. S.& Perera, Hemamali. Development and Validation of a Simple High Performance Liquid ChromatographyUV Method for Simultaneous Determination of Urinary Uric Acid, Hypoxanthine, and Creatinine in Human Urine. International Journal of Analytical Chemistry. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1166297
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1166297
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر