Abnormal Uroguanylin Immunoreactive Cells Density in the Duodenum of Patients with Diarrhea-Predominant Irritable Bowel Syndrome Changes following Fecal Microbiota Transplantation
المؤلفون المشاركون
Hausken, Trygve
Mazzawi, Tarek
Eikrem, Øystein
Lied, Gülen Arslan
المصدر
Gastroenterology Research and Practice
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-02-04
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Altered densities of enteroendocrine cells play an important role in patients with irritable bowel syndrome (IBS).
Uroguanylin activates guanylate cyclase-C to regulate intestinal electrolyte and water transport.
Aim.
To quantify uroguanylin immunoreactive cells density in the duodenum of diarrhea-predominant IBS (IBS-D) patients compared to controls and to investigate the effect of fecal microbiota transplantation (FMT) on these cell densities.
Method.
Twelve patients with IBS-D according to Rome III criteria were included.
The cause was identified as post infectious (PI, n=6) or idiopathic (n=6).
They completed the IBS-symptom questionnaire before and 3 weeks after FMT.
Thirty grams of fresh feces donated from healthy relatives were diluted with 60 ml normal saline and instilled via endoscope into the duodenum.
Biopsies were taken from the patients’ duodenum before and 3 weeks after FMT.
Duodenal biopsies taken from eight healthy controls were also included.
The biopsies were immunostained for uroguanylin and quantified using computerized image analysis.
Results.
Uroguanylin immunoreactive cells were found both in duodenal villi and crypts in both controls and IBS-D patients.
The densities of uroguanylin immunoreactive cells were significantly lower in the villi (P<0.0001) and higher in the crypts (P<0.0001) for the patients than the controls.
Following FMT, the densities of uroguanylin immunoreactive cells for the total group and idiopathic subgroup decreased significantly in the duodenal crypts (P=0.049 and 0.04, respectively) but not in the villi.
No significant changes were shown in the PI-IBS subgroups.
The cells density in only the crypts correlated with diarrhea (r=0.97, P=0.001) and bloating (r=–0.91, P=0.01) in the PI-IBS subgroup before FMT and with abdominal pain (r=0.63, P=0.03) in the total group of IBS-D patients after FMT.
Conclusion.
Altered uroguanylin immunoreactive cells density was found in IBS-D patients compared to controls.
Changes in these cells density following FMT correlated with IBS symptoms (diarrhea, bloating, and abdominal pain).
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mazzawi, Tarek& Eikrem, Øystein& Lied, Gülen Arslan& Hausken, Trygve. 2020. Abnormal Uroguanylin Immunoreactive Cells Density in the Duodenum of Patients with Diarrhea-Predominant Irritable Bowel Syndrome Changes following Fecal Microbiota Transplantation. Gastroenterology Research and Practice،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1166766
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mazzawi, Tarek…[et al.]. Abnormal Uroguanylin Immunoreactive Cells Density in the Duodenum of Patients with Diarrhea-Predominant Irritable Bowel Syndrome Changes following Fecal Microbiota Transplantation. Gastroenterology Research and Practice No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1166766
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mazzawi, Tarek& Eikrem, Øystein& Lied, Gülen Arslan& Hausken, Trygve. Abnormal Uroguanylin Immunoreactive Cells Density in the Duodenum of Patients with Diarrhea-Predominant Irritable Bowel Syndrome Changes following Fecal Microbiota Transplantation. Gastroenterology Research and Practice. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1166766
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1166766
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر