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Prevalence of Genotypes and Subtypes of Gardnerella vaginalis in South African Pregnant Women
المؤلفون المشاركون
Tinarwo, Partson
Pillay, Kayla
Nzimande, Silondiwe
Naicker, Meleshni
Ramsuran, Veron
Abbai, Nathlee Samantha
المصدر
Infectious Diseases in Obstetrics and Gynecology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-07-02
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Background.
Gardnerella vaginalis, a microorganism highly linked to bacterial vaginosis (BV), is understudied in terms of genotypic heterogeneity in South African populations.
This study investigated the prevalence of G.
vaginalis genotypes in BV-positive, BV-intermediate, and BV-negative South African pregnant women.
Methods.
The study population included n=354 pregnant women recruited from a public hospital in Durban, South Africa.
The women provided self-collected vaginal swabs for BV diagnosis by Nugent scoring.
For the genotyping assays, the 16S rRNA and sialidase A genes from BV-negative, BV-intermediate, and BV-positive samples were amplified with G.
vaginalis-specific primers.
The16S rRNA amplicon was digested with TaqI to generate genotyping profiles, and subtypes were determined by correlating BamHI and HindIII digestion profiles.
Phylogenetic analysis was performed on the 16S rRNA and sialidase A sequences.
The data analysis was performed with R Statistical Computing software, version 3.6.2.
Results.
Two different genotypes, GT1 and GT2, were detected.
The most prevalent genotype was GT1.
Four subtypes (1, 2B, 2AB, and 2C) were shown to be present.
The most prevalent subtype was 2B, followed by subtypes 1, 2C, and 2AB.
The phylogenetic analysis of the 16S rRNA showed the presence of 5 clusters.
The tree displayed clusters which contained sequences from the same BV group with different genotypes and subtypes.
Clusters with sequences from across the BV groups carrying the same genotype and subtype were present.
Diversity of the sialidase A across BV groups and genotypes was observed.
Finally, the study did not find a significant association (p>0.05) between reported symptoms of abnormal vaginal discharge and genotype harboured.
Conclusion.
This study provided the first report on the diversity of G.
vaginalis in South African pregnant women.
Diversity assessments of G.
vaginalis with respect to genotypes and virulence factors may aid in a greater understanding of the pathogenesis of this microorganism.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pillay, Kayla& Nzimande, Silondiwe& Naicker, Meleshni& Ramsuran, Veron& Tinarwo, Partson& Abbai, Nathlee Samantha. 2020. Prevalence of Genotypes and Subtypes of Gardnerella vaginalis in South African Pregnant Women. Infectious Diseases in Obstetrics and Gynecology،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1167264
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pillay, Kayla…[et al.]. Prevalence of Genotypes and Subtypes of Gardnerella vaginalis in South African Pregnant Women. Infectious Diseases in Obstetrics and Gynecology No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1167264
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pillay, Kayla& Nzimande, Silondiwe& Naicker, Meleshni& Ramsuran, Veron& Tinarwo, Partson& Abbai, Nathlee Samantha. Prevalence of Genotypes and Subtypes of Gardnerella vaginalis in South African Pregnant Women. Infectious Diseases in Obstetrics and Gynecology. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1167264
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1167264
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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