RP-HPLC Method Development and Validation of Synthesized Codrug in Combination with Indomethacin, Paracetamol, and Famotidine

المؤلفون المشاركون

Abualhasan, Murad N.
Assali, Mohyeddin
Zohud, Nihal
Ghazal, Noura

المصدر

International Journal of Analytical Chemistry

العدد

المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2020-07-01

دولة النشر

مصر

عدد الصفحات

9

التخصصات الرئيسية

الكيمياء
علوم

الملخص EN

Background.

Indomethacin is considered a potent nonsteroidal anti-inflammatory drug that could be combined with Paracetamol to have superior and synergist activity to manage pain and inflammation.

To reduce the gastric side effect, they could be combined with Famotidine.

Methodology.

A codrug of Indomethacin and Paracetamol was synthesized and combined in solution with Famotidine.

The quantification of the pharmaceutically active ingredients is pivotal in the development of pharmaceutical formulations.

Therefore, a novel reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated according to the International Council for Harmonization (ICH) Q2R1 guidelines.

A reverse phase C18 column with a mobile phase acetonitrile: sodium acetate buffer 60 : 40 at a flow rate of 1.4 mL/min and pH 5 was utilized.

Results.

The developed method showed good separation of the four tested drugs with a linear range of 0.01–0.1 mg/mL (R2 > 0.99).

The LODs for FAM, PAR, IND, and codrug were 3.076 × 10−9, 3.868 × 10−10, 1.066 × 10−9, and 4.402 × 10−9 mg/mL respectively.

While the LOQs were 9.322 × 10−9, 1.172 × 10−10, 3.232 × 10−9, and 1.334 × 10−8 mg/mL, respectively.

Furthermore, the method was precise, accurate, selective, and robust with values of relative standard deviation (RSD) less than 2%.

Moreover, the developed method was applied to study the in vitro hydrolysis and conversion of codrug into Indomethacin and Paracetamol.

Conclusion.

The codrug of Indomethacin and Paracetamol was successfully synthesized for the first time.

Moreover, the developed analytical method, to our knowledge, is the first of its kind to simultaneously quantify four solutions containing the following active ingredients of codrug, Indomethacin, Paracetamol, and Famotidine mixture with added pharmaceutical inactive ingredients in one HPLC run.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Assali, Mohyeddin& Abualhasan, Murad N.& Zohud, Nihal& Ghazal, Noura. 2020. RP-HPLC Method Development and Validation of Synthesized Codrug in Combination with Indomethacin, Paracetamol, and Famotidine. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167593

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Abualhasan, Murad N.…[et al.]. RP-HPLC Method Development and Validation of Synthesized Codrug in Combination with Indomethacin, Paracetamol, and Famotidine. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1167593

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Assali, Mohyeddin& Abualhasan, Murad N.& Zohud, Nihal& Ghazal, Noura. RP-HPLC Method Development and Validation of Synthesized Codrug in Combination with Indomethacin, Paracetamol, and Famotidine. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167593

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1167593