Molecular Identification of Aminoglycoside-Modifying Enzymes and Plasmid-Mediated Quinolone Resistance Genes among Klebsiella pneumoniae Clinical Isolates Recovered from Egyptian Patients
المؤلفون المشاركون
Ashour, Mohammed Seif El-Din
Shohayeb, Mohamed
El-Badawy, Mohamed F.
Tawakol, Wael M.
El-Far, Shaymaa W.
Maghrabi, Ibrahim A.
Al-Ghamdi, Saleh A.
Mansy, Moselhy S.
المصدر
International Journal of Microbiology
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-05-30
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Inappropriate use of antibiotics in clinical settings is thought to have led to the global emergence and spread of multidrug-resistant pathogens.
The goal of this study was to investigate the prevalence of genes encoding aminoglycoside resistance and plasmid-mediated quinolone resistance among clinical isolates of Klebsiella pneumoniae.
All K.
pneumoniae isolates were phenotypically identified using API 20E and then confirmed genotypically through amplification of the specific K.
pneumoniae phoE gene.
All isolates were genotyped by the enterobacterial repetitive intergenic consensus polymerase chain reaction technique (ERIC-PCR).
Antibiotic susceptibility testing was done by a modified Kirby-Bauer method and broth microdilution.
All resistant or intermediate-resistant isolates to either gentamicin or amikacin were screened for 7 different genes encoding aminoglycoside-modifying enzymes (AMEs).
In addition, all resistant or intermediate-resistant isolates to either ciprofloxacin or levofloxacin were screened for 5 genes encoding the quinolone resistance protein (Qnr), 1 gene encoding quinolone-modifying enzyme, and 3 genes encoding quinolone efflux pumps.
Biotyping using API 20E revealed 13 different biotypes.
Genotyping demonstrated that all isolates were related to 2 main phylogenetic groups.
Susceptibility testing revealed that carbapenems and tigecycline were the most effective agents.
Investigation of genes encoding AMEs revealed that acc(6′)-Ib was the most prevalent, followed by acc(3′)-II, aph(3′)-IV, and ant(3′′)-I.
Examination of genes encoding Qnr proteins demonstrated that qnrB was the most prevalent, followed by qnrS, qnrD, and qnrC.
It was found that 61%, 26%, and 12% of quinolone-resistant K.
pneumoniae isolates harbored acc(6′)-Ib-cr, oqxAB, and qebA, respectively.
The current study demonstrated a high prevalence of aminoglycoside and quinolone resistance genes among clinical isolates of K.
pneumoniae.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
El-Badawy, Mohamed F.& Tawakol, Wael M.& El-Far, Shaymaa W.& Maghrabi, Ibrahim A.& Al-Ghamdi, Saleh A.& Mansy, Moselhy S.…[et al.]. 2017. Molecular Identification of Aminoglycoside-Modifying Enzymes and Plasmid-Mediated Quinolone Resistance Genes among Klebsiella pneumoniae Clinical Isolates Recovered from Egyptian Patients. International Journal of Microbiology،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1167645
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
El-Badawy, Mohamed F.…[et al.]. Molecular Identification of Aminoglycoside-Modifying Enzymes and Plasmid-Mediated Quinolone Resistance Genes among Klebsiella pneumoniae Clinical Isolates Recovered from Egyptian Patients. International Journal of Microbiology No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1167645
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
El-Badawy, Mohamed F.& Tawakol, Wael M.& El-Far, Shaymaa W.& Maghrabi, Ibrahim A.& Al-Ghamdi, Saleh A.& Mansy, Moselhy S.…[et al.]. Molecular Identification of Aminoglycoside-Modifying Enzymes and Plasmid-Mediated Quinolone Resistance Genes among Klebsiella pneumoniae Clinical Isolates Recovered from Egyptian Patients. International Journal of Microbiology. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1167645
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1167645
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر