Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic Study
المؤلفون المشاركون
Zhang, Shuo-Feng
Wei, Mengmeng
Wu, Siyang
Kang, Chen
Li, Yingfei
Cheng, Wenhao
Li, Yinghui
Yang, Wei
Gao, Yang
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-03-03
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Radix Polygoni Multiflori (RPM) has been widely used to treat various diseases in Asian countries for many centuries.
Although, stilbenes and anthraquinones, two major components of RPM, show various bioactive effects, it has been speculated that the idiosyncratic hepatotoxicity induced by RPM may be related to these constituents.
However, information on the pharmacokinetics of stilbenes and anthraquinones at a subtoxic dose of RPM is limited.
A simple and sensitive UPLC-MS/MS bioanalytical method for the simultaneous determination of 13 ingredients of RPM, including chrysophanol, emodin, aloe-emodin, rhein, physcion, questin, citreorosein, questinol, 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside, torachrysone-8-O-glucoside, chrysophanol-8-O-β-D-glucoside, emodin-8-O-β-D-glucoside, and physcion-8-O-β-D-glucoside, in rat plasma was established.
Acetonitrile was employed to precipitate the plasma with appropriate sensitivity and acceptable matrix effects.
Chromatographic separation was performed using a waters HSS C18 column with a gradient elution using water and acetonitrile both containing 0.025% formic acid within a run time of 9 min.
The constituents were detected in negative ionization mode using multiple reaction monitoring.
The method was fully validated in terms of selectivity, linearity, accuracy, precision, recovery, matrix effects, and stability.
The lower limit of quantitation of the analytes was 0.1–1 ng/mL.
The intrabatch and interbatch accuracies were 87.1–109%, and the precision was within the acceptable limits.
The method was applied to a pharmacokinetic study after oral administration of RPM extract to rats at a subtoxic dose of 36 g/kg.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Cheng, Wenhao& Li, Yinghui& Yang, Wei& Wu, Siyang& Wei, Mengmeng& Gao, Yang…[et al.]. 2020. Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic Study. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1167648
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Cheng, Wenhao…[et al.]. Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic Study. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1167648
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Cheng, Wenhao& Li, Yinghui& Yang, Wei& Wu, Siyang& Wei, Mengmeng& Gao, Yang…[et al.]. Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic Study. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1167648
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1167648
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر