Fabrication of an Original Transparent PVAGelatin Hydrogel: In Vitro Antimicrobial Activity against Skin Pathogens

الملخص EN

The design of actively efficient and low-toxicity formulations against virulent bacterial strains causing skin infections remains a challenging task.

The aim of the present study was to develop and evaluate in vitro a hydrogel impregnated with a known plant extract for topical applications against major skin bacteria.

A poly(vinyl alcohol) (PVA)/gelatin hydrogel, namely HG, was prepared by esterification following the solution casting method.

The gelling process was realized by cross-linking the synthetic polymer PVA and the biopolymer gelatin in the presence of hydrochloric acid (HCl).

A crude extract of Nigella sativa seeds was then encapsulated in HG, and the resulting HGE was characterized morphologically (by Scanning Electron Microscopy (SEM)), structurally (by X-ray powder diffraction (XRD) and Fourier Transform Infrared (FTIR) spectroscopy), behaviorally (by swelling behavior), and biologically (by the agar well diffusion method).

The results of HGE were compared to HG and HG impregnated with 10% acetic acid (HGAA).

SEM sections of HGE revealed a dense and porous surface, suggesting a good hydrophilicity.

X-ray diffractograms indicated that HGE and HG had a similar degree of crystallinity.

FTIR spectra confirmed that esterification occurred between PVA and gelatin suggesting that the amine group is involved in the intercalation of the plant extract components in HG.

Further, HGE was found to be as wettable and swellable as HG, suggesting a good biocompatibility.

Eventually, HGE exerted a pronounced inhibitory effect against two major skin pathogens, the Gram-negative Pseudomonas aeruginosa and the Gram-positive Staphylococcus aureus, suggesting a good extract release.

Taken together, the experimental data indicated that HGE might be a promising wound dressing.