Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix
المؤلفون المشاركون
Togami, Kohei
Ozaki, Hiroaki
Yumita, Yuki
Kitayama, Anri
Tada, Hitoshi
Chono, Sumio
المصدر
International Journal of Biomedical Imaging
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-30
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Idiopathic pulmonary fibrosis is a progressive, chronic lung disease characterized by the accumulation of extracellular matrix proteins, including collagen and elastin.
Imaging of extracellular matrix in fibrotic lungs is important for evaluating its pathological condition as well as the distribution of drugs to pulmonary focus sites and their therapeutic effects.
In this study, we compared techniques of staining the extracellular matrix with optical tissue-clearing treatment for developing three-dimensional imaging methods for focus sites in pulmonary fibrosis.
Mouse models of pulmonary fibrosis were prepared via the intrapulmonary administration of bleomycin.
Fluorescent-labeled tomato lectin, collagen I antibody, and Col-F, which is a fluorescent probe for collagen and elastin, were used to compare the imaging of fibrotic foci in intact fibrotic lungs.
These lung samples were cleared using the ClearT2 tissue-clearing technique.
The cleared lungs were two dimensionally observed using laser-scanning confocal microscopy, and the images were compared with those of the lung tissue sections.
Moreover, three-dimensional images were reconstructed from serial two-dimensional images.
Fluorescent-labeled tomato lectin did not enable the visualization of fibrotic foci in cleared fibrotic lungs.
Although collagen I in fibrotic lungs could be visualized via immunofluorescence staining, collagen I was clearly visible only until 40 μm from the lung surface.
Col-F staining facilitated the visualization of collagen and elastin to a depth of 120 μm in cleared lung tissues.
Furthermore, we visualized the three-dimensional extracellular matrix in cleared fibrotic lungs using Col-F, and the images provided better visualization than immunofluorescence staining.
These results suggest that ClearT2 tissue-clearing treatment combined with Col-F staining represents a simple and rapid technique for imaging fibrotic foci in intact fibrotic lungs.
This study provides important information for imaging various organs with extracellular matrix-related diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Togami, Kohei& Ozaki, Hiroaki& Yumita, Yuki& Kitayama, Anri& Tada, Hitoshi& Chono, Sumio. 2020. Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix. International Journal of Biomedical Imaging،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1169145
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Togami, Kohei…[et al.]. Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix. International Journal of Biomedical Imaging No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1169145
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Togami, Kohei& Ozaki, Hiroaki& Yumita, Yuki& Kitayama, Anri& Tada, Hitoshi& Chono, Sumio. Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix. International Journal of Biomedical Imaging. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1169145
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1169145
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر