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High-Affinity Detection of Alpha-Synuclein by Aptamer-Gold Conjugates on an Amine-Modified Dielectric Surface
المؤلفون المشاركون
Gopinath, Subash C. B.
Lakshmipriya, Thangavel
You, Xuemei
Li, Dingan
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-30
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Parkinson’s disease (PD) is a progressive health issue and influences an increasingly larger number of people, especially at older ages, affecting the central nervous system (CNS).
Alpha-synuclein is a biomarker closely correlated with the CNS and PD.
The loss of neuronal cells in the substantia nigra leads to the aggregation of alpha-synuclein in the form of Lewy bodies, and Lewy neuritis is a neuropathological hallmark.
The therapeutic approach of PD focuses on alpha-synuclein as an important substrate of PD pathology.
So far, research has focused on antialpha-synuclein to minimize the burden of extracellular alpha-synuclein in the brain, and as a consequence, it ameliorates inflammation.
Interdigitated electrode (IDE) biosensors are efficient tools for detecting various analytes and were chosen in this study to detect alpha-synuclein on amine-modified surfaces by using antiaptamer-alpha-synuclein as the probe.
In addition, a gold nanoparticle-conjugated aptamer was used to enhance the detection limit.
The limit of detection for the binding between alpha-synuclein and aptamer was found to be 10 pM.
Control experiments were performed with two closely related proteins, amyloid-beta and tau, to reveal the specificity; the results show that the aptamer only recognized alpha-synuclein.
The proposed strategy helps to identify the binding of aptamer and alpha-synuclein and provides a possible method to lower alpha-synuclein levels and inflammation in PD patients.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
You, Xuemei& Gopinath, Subash C. B.& Lakshmipriya, Thangavel& Li, Dingan. 2019. High-Affinity Detection of Alpha-Synuclein by Aptamer-Gold Conjugates on an Amine-Modified Dielectric Surface. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1169231
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
You, Xuemei…[et al.]. High-Affinity Detection of Alpha-Synuclein by Aptamer-Gold Conjugates on an Amine-Modified Dielectric Surface. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1169231
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
You, Xuemei& Gopinath, Subash C. B.& Lakshmipriya, Thangavel& Li, Dingan. High-Affinity Detection of Alpha-Synuclein by Aptamer-Gold Conjugates on an Amine-Modified Dielectric Surface. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1169231
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1169231
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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