A Sensitive and Efficient Method for Determination of Capecitabine and Its Five Metabolites in Human Plasma Based on One-Step Liquid-Liquid Extraction
المؤلفون المشاركون
Zhang, Feng
Li, Mingming
Gao, Shouhong
Chen, Wansheng
Wang, Zhipeng
Li, Xinxing
Yang, Yang
Chen, Wei
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-01-03
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Colorectal cancer is the most common critical disease both in the developed and developing countries.
Capecitabine, which has served in clinical practice at least for 10 years, is a first-line antidigestive tract cancer drug for its better efficacy, patient compliance, and lower side effects.
An ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method has been developed and completely validated for simultaneous determination of capecitabine and its five metabolites in human plasma from colorectal cancer patients after administration of capecitabine tablet.
One-step liquid-liquid extraction was successfully applied using ethyl acetate and isopropanol (19 : 1, V : V) for sample pretreatment.
Chromatographic separation was achieved within 5 min based on an Atlantis T3-C18 column (3.0 µm, 2.1 × 100 mm) with gradient elution using mobile phases consisting of 0.0075% formic acid in water (pH 4) and in acetonitrile, and the flow rate was 0.3 mL/min.
Linear range was approximately 20.0–5000.0 ng/mL for all analytes.
Linear correlation coefficients were >0.99 for all regression curves.
The intraday and interday accuracy and precision of the method were within ±15.0% and less than 15.0%, respectively.
The mean recovery and matrix effect as well as stability of all the analytes ranged from 59.27% to 90.15% and from 74.84% to 114.48% as well as within ±15.0%.
This simple, rapid, and sensitive method was successfully applied in 42 sparse clinical samples to verify its practicability.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Zhipeng& Li, Xinxing& Yang, Yang& Zhang, Feng& Li, Mingming& Chen, Wei…[et al.]. 2019. A Sensitive and Efficient Method for Determination of Capecitabine and Its Five Metabolites in Human Plasma Based on One-Step Liquid-Liquid Extraction. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1169362
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Zhipeng…[et al.]. A Sensitive and Efficient Method for Determination of Capecitabine and Its Five Metabolites in Human Plasma Based on One-Step Liquid-Liquid Extraction. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1169362
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Zhipeng& Li, Xinxing& Yang, Yang& Zhang, Feng& Li, Mingming& Chen, Wei…[et al.]. A Sensitive and Efficient Method for Determination of Capecitabine and Its Five Metabolites in Human Plasma Based on One-Step Liquid-Liquid Extraction. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1169362
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1169362
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر