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Stable Isotope-Resolved Metabolomic Differences between Hormone-Responsive and Triple-Negative Breast Cancer Cell Lines
المؤلفون المشاركون
Winnike, Jason H.
Stewart, Delisha A.
Pathmasiri, Wimal W.
McRitchie, Susan L.
Sumner, Susan J.
المصدر
International Journal of Breast Cancer
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-09-30
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Purpose.
To conduct an exploratory study to identify mechanisms that differentiate Luminal A (BT474 and MCF-7) and triple-negative (MDA-MB-231 and MDA-MB-468) breast cancer (BCa) cell lines to potentially provide novel therapeutic targets based on differences in energy utilization.
Methods.
Cells were cultured in media containing either [U-13C]-glucose or [U-13C]-glutamine for 48 hours.
Conditioned media and cellular extracts were analyzed by 1H and 13C NMR spectroscopy.
Results.
MCF-7 cells consumed the most glucose, producing the most lactate, demonstrating the greatest Warburg effect-associated energy utilization.
BT474 cells had the highest tricarboxylic acid cycle (TCA) activity.
The majority of energy utilization patterns in MCF-7 cells were more similar to MDA-MB-468 cells, while the patterns for BT474 cells were more similar to MDA-MB-231 cells.
Compared to the Luminal A cell lines, TNBC cell lines consumed more glutamine and less glucose.
BT474 and MDA-MB-468 cells produced high amounts of 13C-glycine from media [U-13C]-glucose which was integrated into glutathione, indicating de novo synthesis.
Conclusions.
Stable isotopic resolved metabolomics using 13C substrates provided mechanistic information about energy utilization that was difficult to interpret using 1H data alone.
Overall, cell lines that have different hormone receptor status have different energy utilization requirements, even if they are classified by the same clinical BCa subtype; and these differences offer clues about optimizing treatment strategies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Winnike, Jason H.& Stewart, Delisha A.& Pathmasiri, Wimal W.& McRitchie, Susan L.& Sumner, Susan J.. 2018. Stable Isotope-Resolved Metabolomic Differences between Hormone-Responsive and Triple-Negative Breast Cancer Cell Lines. International Journal of Breast Cancer،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1169431
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Winnike, Jason H.…[et al.]. Stable Isotope-Resolved Metabolomic Differences between Hormone-Responsive and Triple-Negative Breast Cancer Cell Lines. International Journal of Breast Cancer No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1169431
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Winnike, Jason H.& Stewart, Delisha A.& Pathmasiri, Wimal W.& McRitchie, Susan L.& Sumner, Susan J.. Stable Isotope-Resolved Metabolomic Differences between Hormone-Responsive and Triple-Negative Breast Cancer Cell Lines. International Journal of Breast Cancer. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1169431
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1169431
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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