Concordance between Immunohistochemistry and Microarray Gene Expression Profiling for Estrogen Receptor, Progesterone Receptor, and HER2 Receptor Statuses in Breast Cancer Patients in Lebanon
المؤلفون المشاركون
Mukherji, Deborah
Boulos, Fouad I.
Fakhri, Ghina
Akel, Reem
Khalil, Maya K.
Tfayli, Arafat
المصدر
International Journal of Breast Cancer
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-05-31
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Introduction.
Accurate evaluation of estrogen and progesterone receptors and HER2 is critical when diagnosing invasive breast cancer for optimal treatment.
The current evaluation method is via immunohistochemistry (IHC).
In this paper, we compared results of ER, PR, and HER2 from microarray gene expression to IHC in 81 fresh breast cancer specimens.
Methods.
Gene expression profiling was performed using the GeneChip Human Genome U133 Plus 2.0 arrays (Affymetrix Inc).
Immunohistochemical staining for estrogen receptor, progesterone receptor, and HER2 status was performed using standard methods at a CAP-accredited pathology laboratory.
Concordance rates, agreement measures, and kappa scores were calculated for both methods.
Results.
For ER, Kappa score was 0.918 (95% CI, 0.77.3–1.000) and concordance rate was 97.5% (95% CI, 91.4%–99.7%).
For PR, Kappa score was 0.652 (95% CI, 0.405–0.849) and concordance rate was 86.4% (95% CI, 77%–93%).
For HER2, Kappa score was 0.709 (95% CI, 0.428–0.916) and concordance rate was 97.5% (95% CI, 91.4%–99.7%).
Conclusion.
Our results are in line with the available evidence with the concordance rate being the lowest for the progesterone receptor.
In general, microarray gene expression and IHC proved to have high concordance rates.
Several factors can increase the discordance rate such as differences in sample processing.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Fakhri, Ghina& Akel, Reem& Khalil, Maya K.& Mukherji, Deborah& Boulos, Fouad I.& Tfayli, Arafat. 2018. Concordance between Immunohistochemistry and Microarray Gene Expression Profiling for Estrogen Receptor, Progesterone Receptor, and HER2 Receptor Statuses in Breast Cancer Patients in Lebanon. International Journal of Breast Cancer،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1169472
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Fakhri, Ghina…[et al.]. Concordance between Immunohistochemistry and Microarray Gene Expression Profiling for Estrogen Receptor, Progesterone Receptor, and HER2 Receptor Statuses in Breast Cancer Patients in Lebanon. International Journal of Breast Cancer No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1169472
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Fakhri, Ghina& Akel, Reem& Khalil, Maya K.& Mukherji, Deborah& Boulos, Fouad I.& Tfayli, Arafat. Concordance between Immunohistochemistry and Microarray Gene Expression Profiling for Estrogen Receptor, Progesterone Receptor, and HER2 Receptor Statuses in Breast Cancer Patients in Lebanon. International Journal of Breast Cancer. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1169472
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1169472
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر