Cryo-Imaging and Software Platform for Analysis of Molecular MR Imaging of Micrometastases

المؤلفون المشاركون

Wilson, David L.
Qutaish, Mohammed
Zhou, Zhuxian
Prabhu, David
Liu, Yiqiao
Busso, Mallory R.
Izadnegahdar, Donna
Gargesha, Madhusudhana
Lu, Hong
Lu, Zheng-Rong

المصدر

International Journal of Biomedical Imaging

العدد

المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-16، 16ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2018-04-01

دولة النشر

مصر

عدد الصفحات

16

التخصصات الرئيسية

الطب البشري

الملخص EN

We created and evaluated a preclinical, multimodality imaging, and software platform to assess molecular imaging of small metastases.

This included experimental methods (e.g., GFP-labeled tumor and high resolution multispectral cryo-imaging), nonrigid image registration, and interactive visualization of imaging agent targeting.

We describe technological details earlier applied to GFP-labeled metastatic tumor targeting by molecular MR (CREKA-Gd) and red fluorescent (CREKA-Cy5) imaging agents.

Optimized nonrigid cryo-MRI registration enabled nonambiguous association of MR signals to GFP tumors.

Interactive visualization of out-of-RAM volumetric image data allowed one to zoom to a GFP-labeled micrometastasis, determine its anatomical location from color cryo-images, and establish the presence/absence of targeted CREKA-Gd and CREKA-Cy5.

In a mouse with >160 GFP-labeled tumors, we determined that in the MR images every tumor in the lung >0.3 mm2 had visible signal and that some metastases as small as 0.1 mm2 were also visible.

More tumors were visible in CREKA-Cy5 than in CREKA-Gd MRI.

Tape transfer method and nonrigid registration allowed accurate (<11 μm error) registration of whole mouse histology to corresponding cryo-images.

Histology showed inflammation and necrotic regions not labeled by imaging agents.

This mouse-to-cells multiscale and multimodality platform should uniquely enable more informative and accurate studies of metastatic cancer imaging and therapy.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Qutaish, Mohammed& Zhou, Zhuxian& Prabhu, David& Liu, Yiqiao& Busso, Mallory R.& Izadnegahdar, Donna…[et al.]. 2018. Cryo-Imaging and Software Platform for Analysis of Molecular MR Imaging of Micrometastases. International Journal of Biomedical Imaging،Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1169527

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Qutaish, Mohammed…[et al.]. Cryo-Imaging and Software Platform for Analysis of Molecular MR Imaging of Micrometastases. International Journal of Biomedical Imaging No. 2018 (2018), pp.1-16.
https://search.emarefa.net/detail/BIM-1169527

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Qutaish, Mohammed& Zhou, Zhuxian& Prabhu, David& Liu, Yiqiao& Busso, Mallory R.& Izadnegahdar, Donna…[et al.]. Cryo-Imaging and Software Platform for Analysis of Molecular MR Imaging of Micrometastases. International Journal of Biomedical Imaging. 2018. Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1169527

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1169527