In Vitro Culture of Single Bovine Embryos with Microwell Plates Made of Poly(dimethylsiloxane)‎ Cured under Low Pressure

المؤلفون المشاركون

Iwamoto, Daisaku
Kato, Nobuhiro
Taniguchi, Shunji
Taguchi, Yoshitomo
Kishi, Masao
Saeki, Kazuhiro

المصدر

International Journal of Biomaterials

العدد

المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-7، 7ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2018-06-06

دولة النشر

مصر

عدد الصفحات

7

التخصصات الرئيسية

الطب البشري

الملخص EN

Single embryo culture is useful for assessing the developmental competence of an embryo in detail.

Recently, a device made of poly(dimethylsiloxane) (PDMS), which is biocompatible and nontoxic, has been widely used for culture various types of cells.

However, PDMS plates are porous, causing the serious osmolality increment of the medium (over 600 mOsm/kg from Day 4 to Day 7).

Here, we report that curing the PDMS under low pressure (LP-PDMS) greatly reduced the porosity, resulting in a constant osmolality of the medium.

The blastocyst rate of single bovine embryos cultured with LP-PDMS microwell (MW) plates was the same as that of group-cultured embryos (25 embryos/50 μl droplet; control, P>0.05).

These results indicate that MWs on a plate made of PDMS cured under low pressure can be successfully used for individual embryo culture.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Iwamoto, Daisaku& Kato, Nobuhiro& Taniguchi, Shunji& Taguchi, Yoshitomo& Kishi, Masao& Saeki, Kazuhiro. 2018. In Vitro Culture of Single Bovine Embryos with Microwell Plates Made of Poly(dimethylsiloxane) Cured under Low Pressure. International Journal of Biomaterials،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1169613

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Iwamoto, Daisaku…[et al.]. In Vitro Culture of Single Bovine Embryos with Microwell Plates Made of Poly(dimethylsiloxane) Cured under Low Pressure. International Journal of Biomaterials No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1169613

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Iwamoto, Daisaku& Kato, Nobuhiro& Taniguchi, Shunji& Taguchi, Yoshitomo& Kishi, Masao& Saeki, Kazuhiro. In Vitro Culture of Single Bovine Embryos with Microwell Plates Made of Poly(dimethylsiloxane) Cured under Low Pressure. International Journal of Biomaterials. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1169613

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1169613