Determination of Epimedin B in Rat Plasma and Tissue by LC-MSMS: Application in Pharmacokinetic and Tissue Distribution Studies
المؤلفون المشاركون
Xu, Shunjun
Yang, Liu
Sun, Shuai
Feng, Qianru
Yu, Jiejing
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-06-01
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
A simple, sensitive, and specific liquid chromatography tandem mass-spectrometric method was developed and validated for the determination of epimedin B in rat plasma and tissue samples.
After being processed with a protein precipitation method, these samples were separated on an Agilent Eclipse XDB-C18 column with an isocratic mobile phase consisting of acetonitrile and 0.1% formic acid aqueous solution (32 : 68, v/v).
The calibration curve of epimedin B was linear over the concentration range from 1 to 500 ng/mL in plasma and tissue homogenate.
The method was then applied to pharmacokinetic and tissue distribution studies after a single oral administration of Herba Epimedii extract to SD rats.
Results showed that epimedin B reached the plasma peak concentration at 0.4 h and the terminal elimination half-life was 1.6 h in rat plasma, and the plasma area under the curve from time zero to infinity (AUC0–∞) was 14.35 μg/L·h.
The concentration distribution of epimedin B in rat tissue was in the following order: liver > ovary > womb > lung > kidney > spleen > heart > brain, indicating that the compound could be widely distributed in rat, and the reproductive system may be the principal target of epimedin B for female rat.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Feng, Qianru& Xu, Shunjun& Yu, Jiejing& Sun, Shuai& Yang, Liu. 2017. Determination of Epimedin B in Rat Plasma and Tissue by LC-MSMS: Application in Pharmacokinetic and Tissue Distribution Studies. Journal of Analytical Methods in Chemistry،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1170290
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Feng, Qianru…[et al.]. Determination of Epimedin B in Rat Plasma and Tissue by LC-MSMS: Application in Pharmacokinetic and Tissue Distribution Studies. Journal of Analytical Methods in Chemistry No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1170290
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Feng, Qianru& Xu, Shunjun& Yu, Jiejing& Sun, Shuai& Yang, Liu. Determination of Epimedin B in Rat Plasma and Tissue by LC-MSMS: Application in Pharmacokinetic and Tissue Distribution Studies. Journal of Analytical Methods in Chemistry. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1170290
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1170290
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر