Magnetic Solid-Phase Extraction Using Fe3O4@SiO2 Magnetic Nanoparticles Followed by UV-Vis Spectrometry for Determination of Paraquat in Plasma and Urine Samples

الملخص EN

A rapid and simple method was optimized and validated for the separation and quantification of paraquat, a frequently used herbicide and a leading cause of fatal poisoning worldwide, at trace levels with UV-Vis spectrophotometry in plasma and urine samples by direct magnetic solid-phase extraction.

Fe3O4@SiO2 nanoparticles (NPs) were used as the magnetic solid-phase extraction agents and the paraquat absorbed on NPs was eluted using NaOH and ascorbic acid.

Upon optimization, paraquat could be extracted and concentrated from various samples by 35-fold.

The linear range, limit of detection (LOD), correlation coefficient (R), and relative standard deviation (RSD) could reach 15.0–400.0 μg/L, 12.2 μg/L, 0.9987, and 0.65% (n=5, c = 40.0 μg/L), respectively.

The Fe3O4@SiO2 NPs could be reused up to five times.

The method was successfully applied to the determination of paraquat in urine and plasma at different hemoperfusion numbers in a local hospital for the patient of paraquat poisoning.

The experiment result could not only enable immediate medical intervention but also benefit patients’ survival.