Fungal-Host Interaction: Curcumin Modulates Proteolytic Enzyme Activity of Candida albicans and Inflammatory Host Response In Vitro
المؤلفون المشاركون
Chen, Emily
Benso, Bruna
Seleem, Dalia
Ferreira, Luiz Eduardo Nunes
Pasetto, Silvana
Pardi, Vanessa
Murata, Ramiro Mendonça
المصدر
International Journal of Dentistry
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-08-15
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Current treatments for Candida albicans infection are limited due to the limited number of antifungal drugs available and the increase in antifungal resistance.
Curcumin is used as a spice, food preservative, flavoring, and coloring agent that has been shown to have many pharmacological activities.
Thus, this study evaluated the modulatory effects of curcumin on major virulence factors associated with the pathogenicity of C.
albicans.
The minimum inhibitory concentration (MIC) of curcumin against C.
albicans (SC5314) was determined.
Biofilm formation was quantified and the proteinase and phospholipase secretion was measured.
The cytotoxicity was tested in oral fibroblast cells.
A cocultured model was used to analyze the gene expression of proinflammatory cytokines (IL-1β, IL-1α, and IL-6) from host cells, as well SAP-1 and PLB-1 by RT-PCR.
The MIC was between 6.25 and 12.5 µM, and the activity of proteinase enzyme was significantly decreased in biofilms treated with curcumin.
However, proteinase gene expression was not downregulated after curcumin treatment.
Furthermore, gene expressions of host inflammatory response, IL-1β and IL-1α, were significantly downregulated after exposure to curcumin.
In conclusion, curcumin exhibited antifungal activity against C.
albicans and modulated the proteolytic enzyme activities without downregulating the gene expression.
In host inflammatory response, curcumin downregulated IL-1β and IL-1α gene expression.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Emily& Benso, Bruna& Seleem, Dalia& Ferreira, Luiz Eduardo Nunes& Pasetto, Silvana& Pardi, Vanessa…[et al.]. 2018. Fungal-Host Interaction: Curcumin Modulates Proteolytic Enzyme Activity of Candida albicans and Inflammatory Host Response In Vitro. International Journal of Dentistry،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1170436
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Emily…[et al.]. Fungal-Host Interaction: Curcumin Modulates Proteolytic Enzyme Activity of Candida albicans and Inflammatory Host Response In Vitro. International Journal of Dentistry No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1170436
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Emily& Benso, Bruna& Seleem, Dalia& Ferreira, Luiz Eduardo Nunes& Pasetto, Silvana& Pardi, Vanessa…[et al.]. Fungal-Host Interaction: Curcumin Modulates Proteolytic Enzyme Activity of Candida albicans and Inflammatory Host Response In Vitro. International Journal of Dentistry. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1170436
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1170436
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر