Molecular Characterization and Evolution Analysis of Two Forms of TLR5 and TLR13 Genes Base on Larimichthys crocea Genome Data
المؤلفون المشاركون
Pan, Hao
Li, Gong
Jiang, Lihua
Pei, Liyi
Liu, Liqin
Liu, Binjian
Lǚ, Zhenming
Hiromasa, Tabata
Ogura, Atsushi
Wang, Ping
المصدر
International Journal of Genomics
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-09
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
TLRs (Toll-like receptors) are essential in host defense against pathogens.
There are two types of TLR5, namely, membrane form of TLR5 (TLR5M) and soluble form of TLR5 (TLR5S), both of which perform a crucial role in flagellin response.
TLR13 is a TLR that localizes to endosomes and recognizes nucleic acids released by internal microorganisms, including viruses, bacteria, and fungi.
Here, the full-length coding sequence (CDS), protein structure, and immune response and subcellular localization of TLR5 (TLR5S) and TLR13 were characterized in large yellow croaker (Larimichthys crocea).
These TLRs share high sequence homology with other ichthyic TLRs, while also having their own characters; qtPCR was determined and the results found that the three genes were constitutively expressed in all examined tissues: TLR5M was highly expressed in the spleen and liver; TLR13 expression was high in the kidney, liver, and spleen.
And TLRs were upregulated following stimulation with Vibrio parahaemolyticus in the liver, spleen, and kidney.
Immunofluorescence staining revealed that TLR5M were localized in the cytoplasm, while TLR5S and TLR13 were in the endosome.
The evolutionary analysis has shown that TLR13 was clustered with TLR11, 19, 20, 21, and 22, while TLR5 and TLR3 were classified into a group; these results suggest that TLRs are vital in the defense of L.
crocea against bacterial infection and further increase our understanding of TLR function in innate immunity in teleosts.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jiang, Lihua& Pei, Liyi& Wang, Ping& Liu, Liqin& Li, Gong& Liu, Binjian…[et al.]. 2020. Molecular Characterization and Evolution Analysis of Two Forms of TLR5 and TLR13 Genes Base on Larimichthys crocea Genome Data. International Journal of Genomics،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1171256
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jiang, Lihua…[et al.]. Molecular Characterization and Evolution Analysis of Two Forms of TLR5 and TLR13 Genes Base on Larimichthys crocea Genome Data. International Journal of Genomics No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1171256
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jiang, Lihua& Pei, Liyi& Wang, Ping& Liu, Liqin& Li, Gong& Liu, Binjian…[et al.]. Molecular Characterization and Evolution Analysis of Two Forms of TLR5 and TLR13 Genes Base on Larimichthys crocea Genome Data. International Journal of Genomics. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1171256
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1171256
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر