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Application and Validation of Simple Isocratic HPLC-UV-DAD Method with Dual Wavelength Detection for Ivabradine Determination and Its Application in the Study of Stress Degradation
المؤلفون المشاركون
Nowakowska, Joanna
Pikul, Piotr
Marszałł, Marcin
Ciura, Krzesimir
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-08-07
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Ivabradine is a modern drug that selectively lowers the heart rate, improves cardiac energy balance, and reduces heart’s demands for oxygen and energy.
Due to the chemical nature of ivabradine, which absorbs light at 207 nm and 286 nm, its detection was performed at two wavelengths.
A Knauer C8 column was used to develop the RP-HPLC method for determination of ivabradine.
The proposed method was linear from 5 to 100 µg/ml (r>0.999) for both wavelengths and limits of detection (LOD) and limits of quantification (LOQ) were 0.33 and 1.09 µg/ml for 207 nm and 1.19 and 3.97 µg/ml for 286 nm, respectively.
After validation, the investigated method was applied to a stress degradation study.
Numerous degradation products were formed from ivabradine solutions through alkaline and acid hydrolysis, oxidation, and photolysis.
The largest numbers of degradation products were found in the sample exposed to 24 h radiation and alkaline hydrolysis (eight and six products, resp.).
Finally, the simple method using HPLC-UV-DAD was developed and validated.
Its usefulness for the monitoring of possible degradation products was demonstrated.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nowakowska, Joanna& Pikul, Piotr& Marszałł, Marcin& Ciura, Krzesimir. 2017. Application and Validation of Simple Isocratic HPLC-UV-DAD Method with Dual Wavelength Detection for Ivabradine Determination and Its Application in the Study of Stress Degradation. Journal of Chemistry،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1171473
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nowakowska, Joanna…[et al.]. Application and Validation of Simple Isocratic HPLC-UV-DAD Method with Dual Wavelength Detection for Ivabradine Determination and Its Application in the Study of Stress Degradation. Journal of Chemistry No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1171473
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nowakowska, Joanna& Pikul, Piotr& Marszałł, Marcin& Ciura, Krzesimir. Application and Validation of Simple Isocratic HPLC-UV-DAD Method with Dual Wavelength Detection for Ivabradine Determination and Its Application in the Study of Stress Degradation. Journal of Chemistry. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1171473
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1171473
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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