Flavonoids in Different Parts of Lysimachia clethroides Duby Extracted by Ionic Liquid: Analysis by HPLC and Antioxidant Activity Assay
المؤلفون المشاركون
Kang, Wenyi
Wei, Jinfeng
Cui, Lili
Zhang, Zhi-juan
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-06-13
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
To establish methods for simultaneous determination of isoquercitrin, astragalin in leaves, quercetin, and kaempferol in flowers of Lysimachia clethroides Duby, respectively, the methods were ultrasound-assisted extraction combined with RP-HPLC, and ionic liquid was used as the extraction solvent.
Meanwhile, the antioxidant activity of the different extracts of L.
clethroides was evaluated.
Purospher STAR RP-C18 column (4.6 mm × 250 mm, 5 μm) was used for analysis.
The flow rate was 0.6 mL·min−1, and the column temperature was 25°C.
The detection wavelength was 360 nm.
The mobile phases a and b consisted of acetonitrile-0.4% phosphoric acid (18 : 82, v/v), methanol (A), and 0.4% phosphoric acid (B), respectively.
Linear ranges were 0.068~1.64, 0.060~1.44, 0.0080~0.19, and 0.0077~0.18 μg for isoquercitrin, astragalin, quercetin, and kaempferol, respectively.
The average recoveries of the four constituents were 99.17%, 98.39%, 100.68%, and 98.81%, respectively.
The antioxidant activity of the extracts was detected by DPPH, ABTS, and FRAP.
Under the optimized conditions, all the test solutions showed a certain antioxidant activity and the ionic liquid extracts were better than that of extract of methanol.
Ionic liquid used as the extraction solvent had the potential to extract active ingredients efficiently from L.
clethroides, and this method improved the antioxidant activity with accurate and reliable results.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wei, Jinfeng& Zhang, Zhi-juan& Cui, Lili& Kang, Wenyi. 2017. Flavonoids in Different Parts of Lysimachia clethroides Duby Extracted by Ionic Liquid: Analysis by HPLC and Antioxidant Activity Assay. Journal of Chemistry،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1171477
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wei, Jinfeng…[et al.]. Flavonoids in Different Parts of Lysimachia clethroides Duby Extracted by Ionic Liquid: Analysis by HPLC and Antioxidant Activity Assay. Journal of Chemistry No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1171477
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wei, Jinfeng& Zhang, Zhi-juan& Cui, Lili& Kang, Wenyi. Flavonoids in Different Parts of Lysimachia clethroides Duby Extracted by Ionic Liquid: Analysis by HPLC and Antioxidant Activity Assay. Journal of Chemistry. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1171477
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1171477
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر