JNK Signaling Pathway Suppresses LPS-Mediated Apoptosis of HK-2 Cells by Upregulating NGAL
المؤلفون المشاركون
Han, Mei
Pan, Yuxia
Gao, Mengying
Zhang, Junli
Wang, Fan
المصدر
International Journal of Inflammation
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-04-24
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
Objective.
To explore the role of the c-Jun N-terminal kinase (JNK) signaling pathway in upregulated NGAL expression and its antiapoptotic mechanism in lipopolysaccharide (LPS)-mediated renal tubular epithelial cell injury.
Methods.
In vitro, HK-2 cells were divided into five groups (Con, LPS 1 h, LPS 3 h, LPS 6 h, and LPS 12 h groups) based on the time of LPS (10 μM) treatment.
NGAL and caspase-3 gene expression levels were detected by RT-PCR to assess dynamic changes.
HK-2 cells were pretreated with SP600125 (20 μM) for 2 hours, followed by LPS (10 μM) stimulation for 3 hours.
NGAL and caspase-3 gene expression levels were then determined.
Results.
NGAL mRNA was increased significantly within 6 hours, and caspase-3 mRNA was increased within 3 hours after treatment (P<0.05).
Correlation analysis showed a high correlation between their expression (r = 0.448, P<0.05).
After pretreatment with SP600125, mRNA expression of NGAL in the LPS group was inhibited, while that of caspase-3 was increased significantly.
The NGAL mRNA expression level in the SB + LPS group was decreased significantly compared with that in the LPS group, but it was slightly higher than that in the SP group (∼1.5 times of that in the Con group).
However, caspase-3 mRNA expression was increased significantly in the SB + LPS group (P<0.001) (3.5 times of that in the Con group).
It also showed a significant increase compared with SP and LPS groups (P<0.001 vs.
SB group; P<0.05 vs.
LPS group).
We also found that NGAL and caspase 3 proteins were increased significantly in LPS and SP + LPS groups, but SP600125 decreased the NGAL level by almost 35% and increased the caspase 3 level by 50% in the SP + LPS group compared with the LPS group (P<0.05).
Conclusions.
The JNK signaling pathway inhibits LPS-mediated apoptosis of renal tubular epithelial cells by upregulating NGAL.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Han, Mei& Pan, Yuxia& Gao, Mengying& Zhang, Junli& Wang, Fan. 2020. JNK Signaling Pathway Suppresses LPS-Mediated Apoptosis of HK-2 Cells by Upregulating NGAL. International Journal of Inflammation،Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1171856
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Han, Mei…[et al.]. JNK Signaling Pathway Suppresses LPS-Mediated Apoptosis of HK-2 Cells by Upregulating NGAL. International Journal of Inflammation No. 2020 (2020), pp.1-5.
https://search.emarefa.net/detail/BIM-1171856
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Han, Mei& Pan, Yuxia& Gao, Mengying& Zhang, Junli& Wang, Fan. JNK Signaling Pathway Suppresses LPS-Mediated Apoptosis of HK-2 Cells by Upregulating NGAL. International Journal of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1171856
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1171856
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر