Genetic Diversity and Virulence Factors of S. aureus Isolated from Food, Humans, and Animals
المؤلفون المشاركون
Vences-Velázquez, Amalia
Adame-Gómez, Roberto
Castro-Alarcón, Natividad
Toribio-Jiménez, Jeiry
Pérez-Valdespino, Abigail
Leyva-Vázquez, Marco- Antonio
Ramírez-Peralta, Arturo
المصدر
International Journal of Microbiology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-27
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Staphylococcus aureus is a commensal bacterium in humans and animals able to adapt to multiple environments.
The aim of this study was to compare the genetic diversity and virulence profiles of strains of S.
aureus isolated from food (29 strains), humans (43 strains), and animals (8 strains).
80 lipase-producing strains belonging to a biobank of 360 isolates, identified phenotypically as S.
aureus, were selected.
Confirmation of the species was made by amplifying the spA gene and 80% (64/80) of the strains were confirmed within this species.
The virulence profile of each of the isolates was determined by PCR.
The seA gene coding for enterotoxin A was found in 53.1% of the strains, the saK gene, which codes for Staphylokinase, was amplified in 57.8% of the strains, and, finally, the hlB gene coding for β-Hemolysin was amplified in 17.2%.
The profile of antimicrobial resistance was determined by the Kirby Bauer method showing that the strains from food presented greater resistance to erythromycin (40.7%) and ciprofloxacin (18.5%) while in strains isolated from humans were to erythromycin (48.4%) and clindamycin (21.2%).
Also, in strains from animals, a high resistance to erythromycin was observed (75%).
The frequency of MRSA was 12.5% due to the presence of the mec gene and resistance to cefoxitin.
Of the total strains, 68.7% were typed by PCR-RFLP of the coa gene using the AluI enzyme; derived from this restriction, 17 profiles were generated.
Profile 4 (490 bp, 300 bp) was the most frequent, containing a higher number of strains with a higher number of virulence factors and antimicrobial resistance, which is associated with greater adaptation to different environments.
In this study, a wide genetic diversity of strains of S.
aureus from different foods, humans, and animals was found.
This demonstrates evolution, genetic versatility, and, therefore, the adaptation of this microorganism in different environments.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Adame-Gómez, Roberto& Castro-Alarcón, Natividad& Vences-Velázquez, Amalia& Toribio-Jiménez, Jeiry& Pérez-Valdespino, Abigail& Leyva-Vázquez, Marco- Antonio…[et al.]. 2020. Genetic Diversity and Virulence Factors of S. aureus Isolated from Food, Humans, and Animals. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1171965
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Adame-Gómez, Roberto…[et al.]. Genetic Diversity and Virulence Factors of S. aureus Isolated from Food, Humans, and Animals. International Journal of Microbiology No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1171965
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Adame-Gómez, Roberto& Castro-Alarcón, Natividad& Vences-Velázquez, Amalia& Toribio-Jiménez, Jeiry& Pérez-Valdespino, Abigail& Leyva-Vázquez, Marco- Antonio…[et al.]. Genetic Diversity and Virulence Factors of S. aureus Isolated from Food, Humans, and Animals. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1171965
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1171965
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر