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Antifungal Activity of Thai Cajuput Oil and Its Effect on Efflux-Pump Gene Expression in Fluconazole-Resistant Candida albicans Clinical Isolates
المؤلفون المشاركون
Keereedach, Pitchayaphong
Hrimpeng, Karnjana
Boonbumrung, Khaemaporn
المصدر
International Journal of Microbiology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-04
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Candidiasis caused by the fluconazole-resistant opportunistic pathogen Candida albicans is an intractable clinical problem that threatens immunocompromised or normal individuals.
The most common mechanism of fluconazole resistance in C.
albicans is the failure of cells to accumulate the drug due to increased expression of the efflux proteins encoded by the CDR1, CDR2, and MDR1 genes.
Because the number of current antifungal drugs is limited, it is necessary to develop new therapeutic strategies.
This study aimed to evaluate the antifungal activity of Thai Cajuput oil, its synergism with fluconazole, and its effect on efflux-pump gene expression in fluconazole-resistant C.
albicans clinical isolates.
Thus, we first detected the efflux-pump genes in fourteen resistant strains by PCR.
The frequencies of the CDR1, CDR2, and MDR1 genes were 68.75%, 62.5%, and 87.5%, respectively, and these efflux-pump genes were distributed in three distinct patterns.
Subsequently, the antifungal activity of Thai Cajuput oil was assessed by broth macrodilution and its synergism with fluconazole was evaluated by the checkerboard assay.
The changes in the expression levels of CDR1, CDR2, and MDR1 after treatment with Thai Cajuput oil were analyzed by qRT-PCR.
The MICs and MFCs of Thai Cajuput oil ranged from 0.31 to 1.25 μl/ml and 0.63 to 1.25 μl/ml, respectively, and its activity was defined as fungicidal activity.
The MICs of the combination of Thai Cajuput oil and fluconazole were much lower than the MICs of the individual drugs.
Interestingly, sub-MICs of Thai Cajuput oil significantly reduced the MDR1 expression level in resistant strains P<0.05.
Our study suggests that Thai Cajuput oil can be used to create new potential combination therapies to combat the antifungal resistance of C.
albicans.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Keereedach, Pitchayaphong& Hrimpeng, Karnjana& Boonbumrung, Khaemaporn. 2020. Antifungal Activity of Thai Cajuput Oil and Its Effect on Efflux-Pump Gene Expression in Fluconazole-Resistant Candida albicans Clinical Isolates. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1172193
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Keereedach, Pitchayaphong…[et al.]. Antifungal Activity of Thai Cajuput Oil and Its Effect on Efflux-Pump Gene Expression in Fluconazole-Resistant Candida albicans Clinical Isolates. International Journal of Microbiology No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1172193
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Keereedach, Pitchayaphong& Hrimpeng, Karnjana& Boonbumrung, Khaemaporn. Antifungal Activity of Thai Cajuput Oil and Its Effect on Efflux-Pump Gene Expression in Fluconazole-Resistant Candida albicans Clinical Isolates. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1172193
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1172193
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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