Molecular Detection of Carbapenemase-Encoding Genes in Multidrug-Resistant Acinetobacter baumannii Clinical Isolates in South Africa
المؤلفون المشاركون
Vasaikar, Sandeep
Anane, Yaw Adjei
Okuthe, Grace Emily
Songca, Sandile
Apalata, Teke
المصدر
International Journal of Microbiology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-06-13
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Introduction.
Carbapenem-resistant Acinetobacter baumannii has been responsible for an increasing number of hospital-acquired infections globally.
The study investigated the prevalence of carbapenemase-encoding genes in clinical multidrug-resistant A.
baumannii strains.
Materials and Methods.
A total of 100 nonduplicate multidrug-resistant A.
baumannii strains were cultured from clinical samples obtained from healthcare facilities in the O.
R.
Tambo district.
The strains were confirmed by detecting the intrinsic blaOXA-51-like gene.
Antimicrobial susceptibility testing was performed by VITEK® 2 and autoSCAN-4 systems.
The MIC of imipenem and meropenem was rechecked by E-test.
Colistin MIC was confirmed by the broth microdilution method.
Real-time PCR was performed to investigate the presence of carbapenemase-encoding genes.
Results.
Most strains showed high resistance rates (>80%) to the antibiotics tested.
Resistance to amikacin, tetracycline, and tigecycline were 50%, 64%, and 48%, respectively.
All strains were fully susceptible to colistin.
The blaOXA-51-like was detected in all strains whilst blaOXA-23-like, blaOXA-58-like, blaOXA-24-like, blaIMP-1, blaVIM, and blaNDM-1 were found in 70%, 8%, 5%, 4%, 3%, and 2% of strains, respectively.
None of the tested strains harboured the genes blaSIM and blaAmpC.
The coexistence of blaOXA-23-like, and blaIMP-1 or blaOXA-58-like was detected in 1% and 2% strains, respectively.
A distinct feature of our findings was the coharbouring of the genes blaOXA-23-like, blaOXA-58-like, and blaIMP-1 in 2% strains, and this is the first report in the Eastern Cape Province, South Africa.
The intI1 was carried in 80% of tested strains whilst ISAba1/blaOXA-51-like and ISAba1/blaOXA-23-like were detected in 15% and 40% of the strains, respectively.
The detection of blaOXA-23-like, ISAba1/blaOXA-51-like, ISAba1/blaOXA-23-like, and blaOXA-23-like, blaOXA-58-like, and blaIMP-1 carbapenemases in strains had a significant effect on both imipenem and meropenem MICs.
Conclusions.
Results showed a high level of oxacillinases producing A.
baumannii circulating in our study setting, highlighting the need for local molecular surveillance to inform appropriate management and prevention strategies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Anane, Yaw Adjei& Apalata, Teke& Vasaikar, Sandeep& Okuthe, Grace Emily& Songca, Sandile. 2020. Molecular Detection of Carbapenemase-Encoding Genes in Multidrug-Resistant Acinetobacter baumannii Clinical Isolates in South Africa. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1172265
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Anane, Yaw Adjei…[et al.]. Molecular Detection of Carbapenemase-Encoding Genes in Multidrug-Resistant Acinetobacter baumannii Clinical Isolates in South Africa. International Journal of Microbiology No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1172265
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Anane, Yaw Adjei& Apalata, Teke& Vasaikar, Sandeep& Okuthe, Grace Emily& Songca, Sandile. Molecular Detection of Carbapenemase-Encoding Genes in Multidrug-Resistant Acinetobacter baumannii Clinical Isolates in South Africa. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1172265
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1172265
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر