Prevalence of E. coli ST131 among Uropathogenic E. coli Isolates from Iraqi Patients in Wasit Province, Iraq
المؤلفون المشاركون
Al-Guranie, Dhifar Raa’d
Al-Mayahie, Sareaa Maseer
المصدر
International Journal of Microbiology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-10-20
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The emergence of Escherichia coli sequence type 131 (E.
coli ST131) clone represents a major challenge to public health globally, since this clone is reported as highly virulent and multidrug-resistant, thus making it successfully disseminated worldwide.
In Iraq, there is no previous study dealing with this important clone, so this project was suggested to investigate its presence within uropathogenic E.
coli (UPEC) from Iraqi patients in Wasit Province.
A total of 112 UPEC isolates from cases of acute urinary tract infection (UTI) were analysed for phylogenetic groups by quadruplex PCR; then, these isolates were investigated for E.
coli ST131 clone by both conventional and real-time PCR procedures.
The antibiotic susceptibility test was performed by the disk diffusion method.
The results revealed that, out of 112 UPEC isolates, 38 (33.9%) belonged to phylogroup B2.
For conventional PCR, 92.1% (35/38) of B2 E.
coli isolates were positive for E.
coli ST131, of which 34 were O25b-ST131 strain and 1 was O16-ST131 strain.
However, serogroups O25b and O16 represented 17.1% and 2.8%, respectively.
By RT-PCR assay, 15.1% (17/112) and 44.7% (17/38) of total and B2 E.
coli isolates were confirmed as being E.
coli ST131, respectively.
The highest resistance rates of E.
coli ST131 isolates were against the β-lactams, while low resistance rates were against amikacin, nitrofurantoin, and gentamicin.
Fortunately, all isolates were susceptible to carbapenems.
Moreover, 52.9% (9 out of 17) of E.
coli ST131 isolates were MDR.
In conclusion, the presence of E.
coli ST131 among UPEC isolates from Iraqi patients is confirmed with high resistance to most antimicrobials included in this study.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Al-Guranie, Dhifar Raa’d& Al-Mayahie, Sareaa Maseer. 2020. Prevalence of E. coli ST131 among Uropathogenic E. coli Isolates from Iraqi Patients in Wasit Province, Iraq. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1172393
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Al-Guranie, Dhifar Raa’d& Al-Mayahie, Sareaa Maseer. Prevalence of E. coli ST131 among Uropathogenic E. coli Isolates from Iraqi Patients in Wasit Province, Iraq. International Journal of Microbiology No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1172393
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Al-Guranie, Dhifar Raa’d& Al-Mayahie, Sareaa Maseer. Prevalence of E. coli ST131 among Uropathogenic E. coli Isolates from Iraqi Patients in Wasit Province, Iraq. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1172393
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1172393
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر