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Morphological and Molecular Characterization of Toxigenic Aspergillus flavus from Groundnut Kernels in Kenya
المؤلفون المشاركون
Gichimu, Bernard M.
Okayo, Robert O.
Andika, Darius O.
Dida, Mathews M.
K’Otuto, George O.
المصدر
International Journal of Microbiology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-09-07
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Pathogenesis of Aspergillus flavus on important agricultural products is a key concern on human health due to the synthesis and secretion of the hazardous secondary metabolite, aflatoxin.
This study identified and further characterized aflatoxigenic A.
flavus from groundnuts sampled from sundry shops in Kenya using integrated morphological and molecular approaches.
The groundnuts were plated on potato dextrose agar for isolation and morphological observation of A.
flavus based on macroscopic and microscopic features.
Molecular characterization was done through amplification and comparison of the partial sequence of the ITS1-5.8S-ITS2 region.
The expression analysis of aflR, aflS, aflD, aflP, and aflQ genes in the aflatoxin biosynthesis pathways was conducted to confirm the positive identification of A.
flavus.
The gene expression also aided to delineate toxigenic isolates of A.
flavus from atoxigenic ones.
Morphologically, 18 isolates suspected to be A.
flavus were identified.
Out of these, 14 isolates successfully amplified the 500 bp ITS region of A.
flavus or Aspergillus oryzae, while 4 isolates were not amplified.
All the remaining 14 isolates expressed at least one of the aflatoxigenic genes but only 5 had all the genes expressed.
Partial sequencing revealed that isolates 5, 11, 12, 13, and 15 had 99.2%, 97.6%, 98.4%, 97.5%, and 100% homology, respectively, to the A.
flavus isolate LUOHE, ITS-5.8S-ITS2, obtained from the NCBI database.
The five isolates were accurate identification of atoxigenic A.
flavus.
Precise identification of toxigenic strains of A.
flavus will be useful in establishing control strategies of the fungus in food products.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Okayo, Robert O.& Andika, Darius O.& Dida, Mathews M.& K’Otuto, George O.& Gichimu, Bernard M.. 2020. Morphological and Molecular Characterization of Toxigenic Aspergillus flavus from Groundnut Kernels in Kenya. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1172439
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Okayo, Robert O.…[et al.]. Morphological and Molecular Characterization of Toxigenic Aspergillus flavus from Groundnut Kernels in Kenya. International Journal of Microbiology No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1172439
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Okayo, Robert O.& Andika, Darius O.& Dida, Mathews M.& K’Otuto, George O.& Gichimu, Bernard M.. Morphological and Molecular Characterization of Toxigenic Aspergillus flavus from Groundnut Kernels in Kenya. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1172439
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1172439
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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