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Characterisation of Vibrio Species from Surface and Drinking Water Sources and Assessment of Biocontrol Potentials of Their Bacteriophages
المؤلفون المشاركون
Ateba, Collins Njie
Maje, Mpho Defney
Kaptchouang Tchatchouang, Christ Donald
Manganyi, Madira Coutlyne
Fri, Justine
المصدر
International Journal of Microbiology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-04
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
The aim of this study was to characterise Vibrio species of water samples collected from taps, boreholes, and dams in the North West province, South Africa, and assess biocontrol potentials of their bacteriophages.
Fifty-seven putative Vibrio isolates were obtained on thiosulfate-citrate-bile-salt-sucrose agar and identified using biochemical tests and species-specific PCRs.
Isolates were further characterised based on the presence of virulence factors, susceptibility to eleven antibiotics, and biofilm formation potentials.
Twenty-two (38.60%) isolates were confirmed as Vibrio species, comprising V.
harveyi (45.5%, n = 10), V.
parahaemolyticus (22.7%, n = 5), V.
cholerae (13.6%, n = 3), V.
mimicus (9.1%, n = 2), and V.
vulnificus (9.1%, n = 2).
Three of the six virulent genes screened were positively amplified; four V.
parahaemolyticus possessed the tdh (18.18%) and trh (18.18%) genes, while the zot gene was harboured by 3 V.
cholerae (13.64%) and one V.
mimicus (4.55%) isolate.
Isolates revealed high levels of resistance to cephalothin (95.45%), ampicillin (77.27%), and streptomycin (40.91%), while lower resistances (4.55%–27.27%) were recorded for other antimicrobials.
Sixteen (72.7%) isolates displayed multiple antibiotic-resistant properties.
Cluster analysis of antibiotic resistance revealed a closer relationship between Vibrio isolates from different sampling sites.
The Vibrio species displayed biofilm formation potentials at 37°C (63.6, n = 14), 35°C (50%, n = 11), and 25°C (36.4%, n = 8).
Two phages isolated in this study (vB_VpM_SA3V and vB_VcM_SA3V) were classified as belonging to the family Myoviridae based on electron microscopy.
These were able to lyse multidrug-resistant V.
parahaemolyticus and V.
cholerae strains.
These findings not only indicate the presence of antibiotic-resistant virulent Vibrio species from dam, borehole, and tap water samples that could pose a health risk to humans who either come in contact with or consume water but also present these lytic phages as alternative agents that can be exploited for biological control of these pathogenic strains.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Maje, Mpho Defney& Kaptchouang Tchatchouang, Christ Donald& Manganyi, Madira Coutlyne& Fri, Justine& Ateba, Collins Njie. 2020. Characterisation of Vibrio Species from Surface and Drinking Water Sources and Assessment of Biocontrol Potentials of Their Bacteriophages. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1172452
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Maje, Mpho Defney…[et al.]. Characterisation of Vibrio Species from Surface and Drinking Water Sources and Assessment of Biocontrol Potentials of Their Bacteriophages. International Journal of Microbiology No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1172452
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Maje, Mpho Defney& Kaptchouang Tchatchouang, Christ Donald& Manganyi, Madira Coutlyne& Fri, Justine& Ateba, Collins Njie. Characterisation of Vibrio Species from Surface and Drinking Water Sources and Assessment of Biocontrol Potentials of Their Bacteriophages. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1172452
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1172452
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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