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Molecular Typing of Klebsiella pneumoniae Clinical Isolates by Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction
المؤلفون المشاركون
Sedighi, Parinaz
Zarei, Omid
Karimi, Kiana
Taheri, Mohammad
Karami, Pezhman
Shokoohizadeh, Leili
المصدر
International Journal of Microbiology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-21
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
Aim.
Klebsiella pneumoniae is one of the most important causes of nosocomial infections, including pneumonia, sepsis, and urinary tract infection.
Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) technique is a quick, reliable, and cost-effective method for molecular typing of Enterobacteriaceae family members.
This study aimed to detect genetic relatedness among K.
pneumoniae isolates from hospitals in Hamadan city, using ERIC-PCR technique.
Materials and Methods.
A total of 72 K.
pneumoniae isolates were collected from patients admitted to Besat and Sina hospitals.
After detection and confirmation of K.
pneumonia isolates by chemical and conventional microbiological methods, DNAs were extracted after 24 hours of incubation at 37°C, using the boiling method.
ERIC-PCR technique was carried out, and the ERIC patterns were analyzed by online data analysis service (inslico.ehu.es).
ERIC profiles were compared using Dice method and clustered by UPGMA (unweighted pair group method with arithmetic mean) program.
Also, the samples were evaluated by PCR method for the detection of aerobactin gene within their genome.
Finding.
The genetic relatedness among K.
pneumoniae isolates was studied, and results established the genetic diversity of the clinical isolates by detecting 25 different ERIC types, including 14 common types and 11 unique types.
Also, none of the isolates had aerobactin gene.
Discussion.
The results of this study showed high genetic diversity among K.
pneumoniae strains, indicating the polyclonal distribution of K.
pneumoniae isolates in Hamadan hospitals.
This diversity causes problems for the treatment of infections due to the circulation of diverse K.
pneumoniae clones, which possibly have different antimicrobial susceptibility patterns.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sedighi, Parinaz& Zarei, Omid& Karimi, Kiana& Taheri, Mohammad& Karami, Pezhman& Shokoohizadeh, Leili. 2020. Molecular Typing of Klebsiella pneumoniae Clinical Isolates by Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1172526
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sedighi, Parinaz…[et al.]. Molecular Typing of Klebsiella pneumoniae Clinical Isolates by Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction. International Journal of Microbiology No. 2020 (2020), pp.1-5.
https://search.emarefa.net/detail/BIM-1172526
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sedighi, Parinaz& Zarei, Omid& Karimi, Kiana& Taheri, Mohammad& Karami, Pezhman& Shokoohizadeh, Leili. Molecular Typing of Klebsiella pneumoniae Clinical Isolates by Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1172526
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1172526
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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